恩诺沙星与硫氰酸红霉素对铜绿微囊藻的毒性研究_杨弯弯.pdfVIP

2013,33(10) 1829~1834 China Environmental Science * ,,, (, , 430079) . ,96hEC50 84.6,48.2g/L;,; ;., ,,. Fv/Fm X171.5 A 2013 Toxicity of enrofloxacin and erythromycin thiocyanate on Microcystis aeruginosa. YANG Wan-wan, Hien Vu Thi Thu, * WU Yi-xiao, ZHANG Wei-hao (Hubei Key Laboratory of Biomass-Resource Chemical and Environment Biotechnology, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, China). China Environmental Science, 2013,33(10)1829~1834 Abstract The toxic effects of two antibiotics, enrofloxacin and erythromycin thiocyanate, on the growth and physiology of Microcystis aeruginosa are studied. The results show that both enrofloxacin and erythromycin thiocyanate inhibited the growth of M. aeruginosa , and their 96h-EC50values for M. aeruginosa were 84.6μg/L and 48.2μg/L, respectively. The chlorophyll fluorescence and photosynthetic pigment of Microcystis aeruginosa were also inhibited by the antibiotics, and the inhibition effects increased with the increasing concentrations of enrofloxacin and erythromycin thiocyanate. The soluble protein content of M. aeruginosa was promoted in the presence of low concentrations of antibiotics (0.02mg/L), and was inhibited by high levels of antibiotics (0.05mg/L). The content of lipid peroxidation (malondialdehyde, MDA) presented a strong positive correlation with the levels of antibiotics. The study indicated that enrofloxacin and erythromycin thiocyanate both hindered the photosynthesis process of M. aeruginosa and the synthesis of soluble protein, thus inhibiting the growth of M. aeruginosa . Key words enrofloxacin erythromycin thiocyanate microcystis aeruginosa Fv/Fm photosynthetic pigm