核素药物99Tc.docxVIP

核素药物99Tc【摘要】 目的 采用核素药物99Tc-MDP治疗骨质疏松动物模型，分析对骨转换或促进成骨作用的价值。方法 采用盐酸地塞米松（DX）2mg/kg剂量对制作骨质疏松动物模型的实验兔进行肌肉注射，2次/周，注射8周时，取正常对照组和骨质疏松动物模型组各一组处死，观察动物模型所达程度。同时对骨质疏松动物模型组继续维持（DX）肌肉注射，但剂量改为1次/周。99Tc-MDP治疗组采用与成人相同治疗用量2mg/（5ml·kg）在实验兔骨质疏松模型的基础上治疗16周。治疗16周后，对正常对照组、骨质疏松动物模型组和99Tc-MDP治疗组进行腰椎和右侧股骨头行病理细胞学、骨形态计量分析、骨密度、生物力学、X线摄片、CT摄片、核素骨骼扫描以及治疗后血清ALP指标实验室数据分析。结果 8周后正常对照组和骨质疏松动物模型组进行比较，骨质疏松动物模型组的腰椎和右侧股骨头行病理细胞学表现为明显骨质排列疏松，骨形态计量分析、骨密度、生物力学均下降，血清ALP指标明显升高，骨质疏松动物模型成立。治疗16周后实验室结果显示，99Tc-MDP治疗组与骨质疏松动物模型组比较：股骨头和腰椎的骨密度提高20.17%和26.18%；骨形态计量提高108.65%；骨组织ALP降低223.08%；同时实验兔的骨组织生物力学提高了44.24%和87.10%；核素骨骼扫描也显示示踪剂摄取增加43.65%。病理组织学也提示经治疗成骨细胞有所活跃。但X线摄片、CT摄片仅肉眼观察，骨质疏松对照组与99Tc-MDP治疗组之间的变化不明显。结论 99Tc-MDP治疗对成骨功能或成骨细胞的转换有一定的关系，与加速骨形成或促进成骨功能相符，笔者将进一步进行该方面的探讨。　　【关键词】 99Tc-MDP；骨质疏松动物模型；成骨作用；治疗 Research the value of the 99Tc－MDP for animal model osteogenesis 【Abstract】 Objective In order to reseach the value about the 99Tc－MDP treated osteoporosis for animal model.Methods Use the cony to made the animal model，the cony were divided intonormal、osteoporosis and therapy group.The osteoporosis and therapy group with intramuscular injected dexamethasone 2mg/kg twice a week.At the 8thweeks，compare normal group and osteoporosis group with the bone density，then osteoporosis group was continue intramuscular injected once a week until the test end.The 99Tc－MDPtherapy group was used99Tc－MDP2mg/（5ml·kg） 16 weeks as theadult dosage.At the16thweek，compared thethree groups with lumbarvertebraand the right femoral caput in pathocytology、bone density、osteo vitodynamics、bone morphometry、x-ray、CT、nuclear bone scan and ALP.Results Theosteoporosis group’s lumbarvertebra and the right femoral caput is obviousbone rarefaction and drawdown in bone morphometry、bone density、vitodynamics，and the ALP is obviously rise.The osteoporosis is established.The therapy group lumbarvertebra and caput femoral’s bone density is sublimate 20.71 ％ and 26.18％; the bone morphometry is 108.65%，ALP is low 233.08%; the bone tissue vitodynamic