限制修饰系统cglI赋予钝齿棒杆菌抗噬菌体功能活性解析.PDF

生 物 工 程 学 报 Chin J Biotech 2008, May 25; 24(5): 760-765 journals.im.ac.cn Chinese Journal of Biotechnology ISSN 1000-3061 cjb@im.ac.cn © 2008 Institute of Microbiology, CAS CSM, All rights reserved 研究报告 限制修饰系统cglI 赋予钝齿棒杆菌抗噬菌体功能活性 胡永飞, 李铁民, 杨智勇, 张博, 李玉 辽宁大学生命科学院, 沈阳 110036 摘 要: 为解决氨基酸发酵工业中的噬菌体污染问题, 对cglI 基因复合体在钝齿棒杆菌中的功能活性表达进行研究。通 过PCR 从谷氨酸棒杆菌基因组扩增cglI 基因复合体, 构建重组质粒pJL23-cglI, 转化钝齿棒杆菌T6-13 后得到重组菌株。 定性和定量检测重组菌株的噬菌体抗性。实验结果表明, 携带 cglI 基因复合体的重组钝齿棒杆菌显示了明显的抗噬菌 体功能活性和较广的抗噬菌体谱, 进而证实了 cglI 基因复合体用于构建钝齿棒杆菌抗噬菌体菌株的可行性, 为解决氨 基酸发酵生产中的噬菌体污染问题提供了一种有效方法。 关键词: 限制修饰系统, cglI 基因复合体, 钝齿棒杆菌, 噬菌体, 基因工程菌 Phage Resistance of Corynebacterium crenatum Conferred by the Restriction and Modification System cglI Yongfei Hu, Tiemin Li, Zhiyong Yang, Bo Zhang, and Yu Li College of Life Science, Liaoning University, Shenyang 110036, China Abstract: In order to prevent phage contamination in amino acid fermentation, we introduced the restriction and modification system cglI gene complex into Corynebacterium crenatum and studied their phage-resistance. The cglI gene complex was amplified from Corynebacterium glutamicum by PCR and constructed into pJL23 vector. The recombinant strains were obtained by transformation of the recombinant plasmid pJL23-cglI into C. crenatum. Results showed that the recombinant strains possessed strong phage- resistance activity and broad phage-resistance spectrum, demonstrating the feasibility of using cglI gene complex for construction of phage-resistance recombinant C. crenatum strains and presenting a powerful way to solve the problem of phage contaminatio