2第二章 生物化学检验中的诊断酶学.pptVIP

第二章生物化学检验中的诊断酶学 KEY POINTS 1.List the factors that affect enzyme level in blood. 2.State the methods of enzyme activity measurement, and its effect factors. 3.State the physiological actions, tissue distribution, clinical signification, and methods of analysis of ALT，AST，GGT，LD，ALP，ACP，CK，AMS，LPS and CHE，etc. 4.List the isoenzymes methods of analysis, and discuss the migration of CK. 5.List five additional important enzymes and state their clinical signification. Major Objectives 掌握血浆酶病理性变化的机制；酶活性浓度测定的基本原理；酶活性浓度的测定方法；底物或产物浓度的检测；酶偶联反应法；工具酶与共通反应途径；标本及标本采集和处理因素、试剂及方法学因素、仪器因素的影响及控制；最适测定条件与条件的