DNA的重新排列.ppt

Chapter 17 Rearrangement of DNA 17.1 Introduction 17.2 The mating pathway is triggered by pheromone-receptor interactions17.3 The mating response activates a G protein 17.4 Yeast can switch silent and active loci for mating type 17.5 The MAT locus codes for regulator proteins 17.6 Silent cassettes at HML and HMR are repressed 17.7 Unidirectional transposition is initiated by the recipient MAT locus17.8 Regulation of HO expression 17.9 Trypanosomes switch the VSG frequently during infection17.10 New VSG sequences are generated by gene switching 17.11 VSG genes have an unusual structure 17.12 The bacterial Ti plasmid causes crown gall disease in plants17.13 T-DNA carries genes required for infection 17.14 Transfer of T-DNA resembles bacterial conjugation 17.15 Selection of amplified genomic sequences 17.16 Transfection introduces exogenous DNA into cells 17.17 Genes can be injected into animal eggs 17.18 ES cells can be incorporated into embryonic mice 17.19 Gene targeting allows genes to be replaced or knocked out Amplification refers to the production of additional copies of a chromosomal sequence, found as intrachromosomal or extrachromosomal DNA.Transgenic animals are created by introducing new DNA sequences into the germline via addition to the egg. Amplification refers to the production of additional copies of a chromosomal sequence, found as intrachromosomal or extrachromosomal DNA.Transgenic animals are created by introducing new DNA sequences into the germline via addition to the egg. Figure 17.1 Mating type controls several activities. Figure 17.2 The yeast life cycle proceeds through mating of MATa and MATa haploids to give heterozygous diploids that sporulate to generate haploid spores. Figure 17.3 Either a or a factor/receptor interaction triggers the activation of a G protein, whose bg subunits transduce the signal to the next stage in the pathway. Figure 17.4 The same mating type response is triggered by interaction of either pherom