FLASH快速制备色谱.ppt

Fast preparative chromatography ZZY 2014-03-16 Isolation purification method Distill Recrystallization Extraction Column chromatography ( Crawling boards, Glass column , Instrument through the column ) These conventional separation method ， The most common is column Chromatography ，We talk about is the instrument through the column, also known as flash Fast preparative chromatography--Flash 1978 WCStill such as preparative chromatography chemist proposed the concept of fast ， Compared with the conventional technology in the column chromatography, flash time to complete the separation and purification ， This is the one that the origin of the flash 。 Principles of flash chromatography flash-Structural map Flash advantage Saving time Save effort Province solvent UV Detect The application of flash Flashs Application Flash inverting applications Flash column selection and sample volume silica gel 100~200, 200~300, 300~400 Specification 4g、12g、20g、40g、80g、120g etc. Usually the maximum loading capacity for the column silica gel column of 1/20, i.e., generally up to 20g column 1 g of crude product , In practical applications should be based on the degree of separation of each component and the solubility of the sample to determine the specific amount. Flashs Application Flashs Application Select the sampling methods Wet： The solvent must be less polar eluent solvent or adding percent less polar solvents , Otherwise, the component will be easily eluted . And the amount of solvent should not exceed 10% of the column volume Dry ： Most of the samples we used dry sample ， Controlling the amount of silica is 1 ~2 times the sample . Flash inverting applications Inverted principle Reversed phase silica filler is bonded on a substrate such as C4, C8, C18 non-polar aliphatic chain packing . Non-polar or hydrophobic compounds are strongly retained ， The polarity of the sample is weak reserved ， Faster through the column bed . Flash invert