染料木黄酮对卵巢癌HO论文.docVIP

　　染料木黄酮对卵巢癌HO论文 【关键词】 卵巢肿瘤 Inhibitory effect of genistein on human ovarian cancer cell line HO8910PM in nude mice 【Abstract】AIM: To investigate the effects and possible mechanisms of genistein (GEN), a kind of phytoestrogens, bined an ovarian cancer cells in nude mice. METHODS: Ovarian cancer cell line HO8910PM derived from human ovarian serous adenocarcinoma ice, ized equally into 4 groups: GEN treatment group, DDP treatment group, DDP plus GEN treatment group, and a control group. Four ass of the nude mice orphology of tumor cells icroscope. The expressions of PA and microvascular density (MVD) ined by immunohistochemistry. RESULTS: GEN and DDP effectively inhibited the groan ovarian cancer cells transplanted into nude mice and the inhibitory effect orphological changes in condensation and margination of nuclear chromatin, apoptotic bodies and cell necrosis significantly increased in GEN group and GEN plus DDP group. CONCLUSION: Both DDP and GEN can inhibit the groan ovarian cancer cells in nude mice and exert synergistic antitumor effect. The anticancer mechanism of GEN may involve suppressing the proliferation of ovarian cancer cells and angiogenesis and blocking the blood supply of the tumor. 【Keys; genistein; cisplatin; neovascularization, pathologic; proliferating cell nuclear antigen 【摘要】目的： 观察植物雌激素染料木黄酮（GEN）与顺铂（DDP）联合应用治疗人卵巢癌裸鼠移植瘤的效应及其机制. 方法： 建立20只人卵巢癌裸鼠移植瘤模型，随机分成4组，分别为对照组、GEN组、DDP组及GEN+DDP组. 4 L/L小牛血清的RPMI 1640培养液中，置于37℃， 50 mL/L CO2饱和湿度培养箱内，大量扩增细胞. 将处于对数生长期的细胞制成 2×107/mL悬液，注入裸鼠后背部皮下. 穿刺点距注射部位1.2 cm，每只裸鼠皮下接种1点，每点 0.2 mL，含4×106个细胞. 1.2.2治疗待移植瘤直径长成4 mm左右时（约接种后7 d），采用随机数字法将20只裸鼠随机分成4组，每组5只. GEN组灌胃给予GEN 50 mg/kg d，共30 d；DDP组腹腔注射DDP 5mg/kg d，实验开始连续用1 L/次. 1.2.3观察指标GEN治疗30 d后，脱颈处死各组裸鼠，称体质量，解剖裸鼠. 取移植瘤称质量，计算各组平均瘤质量及抑瘤率（IR），IR＝（1-治疗组平均瘤质量/对照组平均瘤质量）×100％. 按金正均法计算q值来评价GEN与DDP两药合用的效果，q=(EA+B)/［EA+(1-EA)×EB］, EA+B为两药合用时的抑瘤率，EA 和EB为各药单独应用时的抑瘤率. q＜0.85表示两药有拮抗作用，0.85≤q≤1.15表示两药有相加作用，q＞1.15 表示两药有协同作用. 各组裸鼠心、肝、脾、肺、肾等重要脏器及移植瘤，用40 g/L中性甲醛溶液固定，常规石蜡包埋、切片. HE染色，光镜下观察. SP免疫