柔红霉素（DNR）荧光直方图诊断白血病细胞耐药的意义论文.docVIP

　　柔红霉素（DNR）荧光直方图诊断白血病细胞耐药的意义论文 【摘要】目的：建立检测细胞内柔红霉素（DNR）的荧光直方图诊断白血病细胞耐药的方法。材料与方法：选取15例临床耐药的急性髓性白血病（AML），5初治AML进行研究，用流式细胞仪（FCM）检测细胞内DNR的荧光强度，根据其强弱程度划分成耐药区和药敏区，其直方图主峰左移（LSMP）诊断为耐药；直方图右移（RSMP）诊断为药敏。耐药指数（DRI），即R/S（R为耐药细胞数，S为药敏细胞数）≥2为耐药，＜2为药敏。荧光指数（FI）=平均荧光强度×DRI.freel of daunorubicin(DNR) in diagnosis of drug resistance to leukemic cells 【Abstract】Objective：To develop a fluorescent histogram of intracellular daunorubicin(DNR) as a diagnostic method for drug resistance in acute myeloid leukemia(AML).Methods：15 patients ined by floeter(FCM).According to a degree of the intensity, both drug－resistan t and－sensitive zones arked off. Left shift of main peak(LSMP) may be diagnosed as drug resistance, and right shift of main peak(RSMP) as drug sensitivity. R/S stood for drug resistant index(DRI);R for the number of drug resistant cells, and S for drug－sensitive cells. DRI≥ 2 ean fluorescent intensity (MFI) multiplied by DRI. FI≥ 200 meant drug-resistant, eant drug-sensitive.Results：Among 5 patients ary drug-resistant for LSMP,DRI=3.60, FI=367. In 15 patients apping the fluorescent histogram of intracellular DNR, in bination anifestation and a cut off point for diagnosis of the fluorescent intensity of drug-resistant cells, ay in good time find and value a type and a degree of drug resistance to AML. Moreover ,it may provide a basis for e and a method for an observation in drug-resistant reversal treatment as ; Drug resistance; Floeter (FCM); Leukemia Drug resistance in leukemia otherapeutic failure. Its degree and its type otherapeutic scheme for each patient. Still, an urgent problem to solve in clinic is hoely. Daunorubicin (DNR) produces red fluorescence easuring DNR fluorescent intensity in the cells. It inutes that floeter(FCM) could analyse it for thousands of cells and dra that represented the marks of drug resistance to leukemic cells. Hence, in classical and in regroia. 1 MATERIALS AND METHODS 1.1 Patients 15 patients yelogenous leukemia(AML),anifested