生物电化学系统合成甲烷和乙酸案例.docVIP

生物电化学系统还原二氧化碳同时合成甲烷和乙酸* 蒋 永1,3 苏 敏1,2 张 尧1,3 陶 勇1 李大平1** (1 中国科学院成都生物研究所 成都 610064) (2 四川大学生命科学学院 成都 610041) （3中国科学院大学 北京 100049） 摘 要 近年来，采用生物电化学系统还原二氧化碳合成有机物已成为环境微生物学研究热点。本研究构建了具有电活性微生物的生物电化学系统，混合菌群通过电极直接传递或电极电子转化为氢气两种方式获得电子同时产生甲烷和乙酸。在设定阴极电势为-1100 mV时，甲烷生成速率为17.3 mL h-1 L-1, 乙酸生成速率为13.9 mg h-1 L-1, 总库伦效率可达94.0%。生物阴极群落构成包括了醋酸杆菌属(Acetobacterium)、甲烷杆菌属(Methanobacterium)和甲烷微粒菌属(Methanocorpusculum)等功能微生物。该研究表明生物电化学系统中，基于混合菌构建的生物阴极可以将二氧化碳还原为多种有机物，并且其中微生物的电子获得方式也存在多样性。图 4 表 0 参 33 关键词 生物电化学系统；生物阴极; 二氧化碳；甲烷；乙酸 CLC Simultaneously production of methane and acetate from carbon dioxide in bioelectrochemical systems* JIANG Yong1,3，SU Min1,2，ZHANG Yao1,3，TAO Yong1 LI Daping1** （ Chengdu Institute of Biology, Chinese Academy of Science, Chengdu 610041,China）(2 School of Life Sciences, Sichuan University, Chengdu 610064, China) (3University of Chinese Academy of Sciences, Beijing 100049, China) Abstract In recent years, reduction of CO2 to organics with bioelectrical system has been a research area of environmental microbiology. This study describes the performance of bioelectrochemical systems(BESs), based on electrochemically active mixed culture, capable of reducing CO2 to CH4 and CH3COOH via abiotically produced H2 gas and/or direct extracellular electron transfer. CH4 and CH3COOH were produced at rates of 17.3mL h-1 L-1 and 13.9 mg h-1 L-1, respectively (at potential of -1100 mV). The current capture efficiency reached to 94.0% in batch potentiostatic experiments. Microbial characterization revealed that the microbial populations were consisting of Methanobacterium palustre, Methanocorpusculum parvum, and acetogen Acetobacterium sp. These results suggest that mixed culture in BESs show the ability to convert CO2 into various organic cmoupands by accepting electrons in different ways. Keywords Bioelectrochemical systems; Biocathode; Carbon dioxide; Methane; Acetate 生物电化学系统（Bioelectrochemical systems ，BESs)是一种能够实现从污水处理过程中回收电能的新型装置[1, 2]。近年来，BESs已应用于更多的领域，包括重金属回收[