树突状细胞新亚群指南.pdfVIP

A R T I C L E S y g o l Splenic stroma drives mature dendritic cells to o n u m differentiate into regulatory dendritic cells m i e r u t 1,3 2,3 1,3 1 1 1 1 a Minghui Zhang , Hua Tang , Zhenhong Guo , Huazhang An , Xuejun Zhu , Wengang Song , Jun Guo , n / m 1 1 2 1,2 o Xin Huang , Taoyong Chen , Jianli Wang Xuetao Cao c . e r u t The fates of dendritic cells (DCs) after antigen presentation have been studied extensively, but the influence of lymphoid a n. microenvironments on DCs is mostly unknown. Here, using splenic stromal cells to mimic the immune microenvironment, we w w show that contact with stromal cells promoted mature DCs to proliferate in a fibronectin-dependent way and that both stromal w / cell contact and stromal cell–derived transforming growth factor-b induced their differentiation into a new regulatory DC subset. / : p We have identified an in vivo counterpart in the spleen with similar phenotype and functions. These differentiated DCs secreted t t h nitric oxide, which mediated the suppression of T cell proliferation in response to antigen presentation by mature DCs. Thus, our p findings identify an important mechanism by which the microenvironment regulates immune responses. u o r G g n Dendritic cells (DCs) are specialized antigen-presenting cells that are on cell-cell contact and transforming growth factor-b (TGF-b). Com- i h pivotal in immune responses. The different DC subsets and their pared with fully matured DCs, these differentiated DCs (diffDCs) had s i l respective fates have been examined extensively1–5. DCs are commonly lower expression of major histocompatibility compl