乳酸菌的益生及免疫调节作用.pptVIP

乳酸杆菌的益生特性及免疫调节作用 International Journal of Food Microbiology Available online 13 May 2011 Keywords:Lactobacilli Probiotic characteristics Immune modulation In the current study, the probiotic potential of approximately 350 strains of lactic acid bacteria isolated from Korean infant feces and Kimchi was investigated. Common probiotic properties of the bacterial strains, such as acid tolerance, bile tolerance and adhesion to human intestinal epithelial cells (HT-29 cells), were examined. Some strains were found to have immune modulatory and antimicrobial properties. Antagonistic activity against a panel of pathogenic bacteria was found to be strain dependent. To evaluate the immune modulatory activity of the strains, lymphocyte interferon (IFN)-γ secretion was determined in conjunction with cell proliferation. Some strains of Lactobacillus gasseri, L. fermentum and L. plantarum exhibited increased IFN-γ levels and lymphocyte proliferation. To evaluate the effects of these immune modulating lactobacilli on host life span, Caenorhabditis elegans was used as an in vivo model. Nematodes that were supplied heat-killed lactobacilli as a food source exhibited obvious differences in life span compared with those fed Escherichia coli OP50. The mean life span (determined as mean percent survival) of worms fed L. plantarum CJLP133 and L. fermentum LA12 was 13.89% and 13.69% greater, respectively, than that of control nematodes after 21 days(P=0.036 and 0.043, respectively). In addition, some of safety profiles, including hemolytic type, gelatin hydration and degradation of urea, were found to be positive. These newly identified lactobacilli hold promise for use as probiotic agents, feed additives and/or in food applications. A b s t r a c t 研究发现，从韩国婴儿的粪便及泡菜中分离出的乳酸菌大约有350株菌株有益生潜力。益生菌的普遍益生特性主要包括：耐酸性，耐胆盐及对人肠上皮细胞的粘附性。还有一些菌株有免疫调节作用及抗菌作用。一些细菌对致病菌有一定的抵抗作用。为了评估这株菌的免疫调节作用，测定淋巴细胞干扰素（IFN） - γ的分泌与增值。一些发酵乳杆菌和植物乳杆菌会使干扰素（IFN） - γ分泌增加及淋巴细胞的增值。一些安全指标