稳定性同位素探测技术在微生物生态学研究中的应用 - 文献信息中心.pdfVIP

26 5 　　　　　　 Vol.26, No.5 2006 5 ACTA ECOLOGICA SINICA May, 2006 ＊ 葛　源, 贺纪正 , 郑袁明, 张丽梅, 朱永官 ( , 　100085) :(stable isotope probing, SIP), , , , 。 :(microcosm) , (, , (DNA RNA)(PLFA ), 、、、, 。 、, 、、、、。 :;;; :1000-0933(2006)05-1574-09　:Q93.3;Q938.1　:A Stable isotope probing and its applications in microbial ecology ＊ GE Y an,HE Ji-Zheng , ZHENG Y an-Ming,ZHANG Li-Mei, ZHU Yong-G an 　(State key Laboratory of Systems Ecology, Research Center f or Eco-Environmental Sciences, ChineseAcademy of Sciences, Beij ing 100085, China).Acta Ecolog ica Sinica, 2006, 26(5):1574 ～ 15 2. Abstract:Stable isotope probing (SIP), a combination of isotope labeling with molec lar biological approach, is a techniq e that is sed to identify microorganisms in environmental samples, and at the same time, to examine microbial f nctions d ring the biogeochemical processes operating in vario s environmental systems.The method has the potential for wide applications in the f t re, since it can provide ab ndant information abo t microbial interactions and metabolic f nctions in complex comm nities. The rationale of the SIP techniq e is as follows.Environmental samples in sit or in microcosm are exposed to s bstrates labeled with stable isotopes.Somemicroorganisms in these samples can metabolize the stable isotope-enriched s bstrates as their carbon or nitrogen reso rce for growth.The stable isotope assimilated by these microorganisms is then sed to synthesize cell lar components s ch as n cleic acids (DNA and RNA)and phospholipid fatty acids (PLFA ).As a res lt, the microbial identity can be linked to their f nctions by extracting and analyzing these stable isotope-labeled biomarkers in the microbial comm nities.Here,