IL-18增加烧伤休克复苏大鼠中性粒细胞中超氧阴离子的生成杨勇.DOCVIP

IL-18增加烧伤休克复苏大鼠中性粒细胞中超氧阴离子的生成 杨 勇，王晓娟，刘德贵，薛 刚（610083成都成都军区总医院烧伤整形科）Interleukin 18 ，IL-18）以及中性粒细胞的含量。腹腔注射IL-18以及抗大鼠IL-18抗体（anti-rat-IL-18 antibody）后，检测模型大鼠中性粒细胞内超氧自由基以及羟自由基的含量。±0.03）vs （0.15±0.02）,P0.01]以及8-OHdG（8-羟基脱氧鸟苷8-Hydroxy-2-deoxyguanosine; 8-Hydroxydeoxyguanosine）[（605±145）pg/ml vs （152±21）pg/ml,P0.01]的含量均显著高于对照组大鼠，而注射anti-rat-IL-18抗体的大鼠中性粒细胞内超氧阴离子[（0.19±0.03）vs（0.32±0.04）,P0.01]以及8-OHdG的含量均[（198±45）pg/ml vs （524±124）pg/ml,P0.01]显著低于单纯烫伤大鼠。离体条件下，加入IL-18的中性粒细胞在体外超氧阴离子[（0.38±0.02）vs （0.13±0.02）,P0.01]以及8-OHdG [（505±99）pg/ml vs （92±21）pg/ml,P0.01]的含量均显著高于对照组大鼠，而注射anti-rat-IL-18抗体的大鼠中性粒细胞内超氧阴离子[（0.16±0.03）vs（0.29±0.03）,P0.01]以及8-OHdG的含量均[（95±45）pg/ml vs （324±68）pg/ml,P0.01]显著低于单纯烫伤大鼠。结论 在烧伤休克延迟复苏大鼠中，IL-18能够有效的与大鼠体内中性粒细胞结合，促进了烧伤休克延迟复苏大鼠模型中的氧自由基的生成，进一步造成了组织的损伤。 [关键词] IL-18；烧伤休克延迟复苏；超氧离子；羟自由基 [中图法分类号] [文献标志码] A [基金项目] 国家自然科学基金 [通信作者] 薛 刚，电话：（028E-mail：kpardan@163.com IL-18 mediated increase in neutrophil superoxide anion production in delayed fluid resuscitation of burn rats Yong Yang, Xiaojuan Wang, Degui Liu, Yanbiao Zhang, Gangxue (Department of Burn and Plastic Surgery, General Hospital of Chengdu Military Command, Chengdu, Sichuan Procince, 610083,China) [Abstract] Objective This study tested the role of IL-18 mediated increase in neutrophil superoxide anion production in delayed fluid resuscitation of burn rats. Methods The level of Il-18 and the number of neutrophil granulocyte were detected in the delayed fluid resuscitation of male rats after 40% total body surface area burn or shame injury at different time point. Male rats were intraperitoneal injected with IL-18(20ug/kg) and anti-rat-Il-18 antibody(80ug/kg) or isotype control. On day 1 after injury, the productions of neutrophil superoxide anion were detected. Furthermore, neutrophils were cultured in vitro and cultured with IL-18 and anti-rat-IL-18 antibody respectively. And then the productions of neutrophil superoxide anion were detected. Result The level of IL-18 (51.