中国蛤蜊精对小鼠免疫活性观察 - 食品科学.PDFVIP

2012-12-05 中国蛤蜊精对小鼠免疫活性观察 1 1 1 1 1 1 2 2 史 倩 ，王晓洁 ，张志伟 ，刘晓晨 ，梁建光 ，王守海 ，刘新生 ，曲彦清 (1.鲁东大学生命科学学院，山东 烟台 264025 ； 2.鲁东大学校医院，山东 烟台 264025) 摘 要：目的：观察中国蛤蜊精（每100g 中，含多糖 19.01g、牛磺酸 1.25g、胆碱 0.65g ）对小鼠免疫功能的影响。方 法：用不同剂量的中国蛤蜊精给小鼠灌胃，连续 10d，测定小鼠白细胞总数及分类计数、通过抗体凝集反应实验测定效 价、测定抗体生成细胞水平、腹腔巨噬细胞吞噬功能、体质量变化及免疫器官重量，并对其结果进行统计分析。结果： 与对照组相比，中国蛤蜊精各剂量组淋巴细胞相对数、抗体生成水平、抗体生成细胞水平、小鼠腹腔巨噬细胞吞噬功能 和脾脏指数均显著升高（P0.05 ）。结论：中国蛤蜊精有显著增强小鼠免疫功能的作用。 关键词：中国蛤蜊精；细胞免疫；体液免疫；非特异性免疫 Observation of Immune Function of the Extract of Mactra chinensis Abstract ：Objective: To observe the immune effect of the extract of Mactra chinensis (EMC: containing 19.01g polysaccharide,1.25g taurine and 0.65g choline every 100g ）on mice. Methods: The mice were orally administered different dosages of EMC for ten days. A series of items related to immune function were analyzed, including the count of quantity and classification of white blood cell (WBC), the titer of antibody production in agglutination reaction and quantity of plaque-forming cell (PFC), phagocytosis test of phagocyte on abdominal, weight of body and immune organs. Results: Contrasted with the control group, the relative number of lymphocytes, activity of antibody production, quantity of antibody forming cells，phagocytic function of mice peritoneal macrophages and the index of spleen of all groups of EMC were improved significantly (P0.05). Conclusion: EMC enhances the immunocompetence of normal mice effectively. Key Words: water extract from Mactra chinensis (EMC); Cellular immunity; Humoral immunity; Nonspecific immunity 中图分类号：TS201.2 文献标识码：A 文章编号087 中国蛤蜊（Mactra chinensis Philippi ）是一种海贝，又名沙蛤、飞蛤，主要分布在我国北方沿海，朝鲜、日本 也有分