不同苗龄假植对烤烟抗氧化系统酶活性影响.doc

不同苗龄假植对烤烟抗氧化系统酶活性影响 　　摘 要：抗氧化系统能够清除植物体内活性氧，是植物提高抗逆性的主要途径，其中，抗氧化系统酶活性起着至关重要的作用。为了筛选出湖南低温寡照烟区烟苗的最适假植期，研究了不同苗龄假植对烤烟整个生育期抗氧化系统酶活性的影响。结果表明，在烤烟整个生育期，SOD，POD，CAT，APX和GR的活性变化趋势均为先升后降；烤烟SOD，CAT，POD，APX和GR的活性最高的时期为打顶期；不同苗龄假植中，5叶1心处理的烤烟抗氧化系统酶活性较高，故其抗逆性较强 关键词：假植；烤烟；抗氧化系统；酶活 中图分类号：S572 文献标识码：A DOI 编码：10.3969/j.issn.1006-6500.2017.04.003 Effect of Transplanting at Different Seedling Ages on Antioxidant System Enzyme Activity of Flue-cured Tobacco CUI Xiaochuang1 ， WANG Xin2， CHEN Yi1， ZHANG Zhen3， XIONG Wei1， YANG Hongqi1 （1. College of Biological Science and Technology，Hunan Agricultural University，Changsha， Hunan 410128， China； 2.China Tobacco Zhejiang Industrial Company Limited， Hangzhou， Zhejiang 550009， China； 3.Shaoyang Tobacco Company of Hunan Province， Shaoyang， Hunan 422000， China） Abstract： Antioxidant system can remove active oxygen in plant， which is the main way for plant to improve stress resistance. And the antioxidant enzyme activity plays a crucial role. To screen out the most suitable transplanting period of tobacco seedlings in Hunan low temperature and less sunlight radiation tobacco-growing areas， this paper studied the effect of transplanting at different seedling ages on antioxidant system enzyme activity of flue-cured tobacco in the whole growing period. The results showed that the change trends of the activities of SOD， POD， CAT， APX and GR of flue-cured tobacco were ‘up-down’ in the whole growing period. The activities of SOD， CAT， POD， APX and GR of flue-cured tobacco were the highest in the topping stage. Antioxidant system enzyme activity of flue-cured tobacco transplanted in five-leaf stage in different seedling ages was high. Therefore， the resistance of flue-cured tobacco in five-leaf stage was stronger. Key words： transplanting； flue-cured tobacco； antioxidant system； enzyme activity 育苗是??草生产中最重要的环节之一，培育整齐健壮、抗性高的烟苗是生产优质、适产烤烟的重要基础[1]。为应对湖南烟区育苗期间“低温寡照”的恶劣气候，水旱两段式育苗在漂浮育苗的基础上加以改进，在漂浮育苗烟苗达到4叶1心时，假植到已装填营养土的托盘里，最后在烟苗达到8叶1心时进行大田移栽，可