双向电泳技术及其在乳酸菌研究中的应用.pdfVIP

川 由 ， 中 盯 业 lLN ⑩ 亿 几 国 子;I 帽 m m 口 哑 工 Z肝gy@163.com 双向电泳技术及其在乳酸菌研究中的应用 王超1 ，曲酶军2 ，崔艳华1 (1.哈尔滨工业大学食品科学与工程学院，哈尔滨150090;2. 黑龙江省科学院微生物研究所，哈尔滨 150010) 摘 要:双向电泳技术是蛋白质纽学研究中最流行的一种技术，是大规模筛选蛋白质差异表达的最佳方法。该技术主妥通过等也聚 焦和SDS相结合的方法，通过等电点和分子量大小对蛋白质进行分离。因其具有方便、快速Ã良好的重复率和分辨率，是蛋 白质组学研究中最广泛应用的技术。本文就其技术产生、原理、影响因素及其在乳酸菌研究中的应用做一综述。 关键词:双向电泳;等电聚焦;SDS;影响因素;应用 中固分类号:Q936 文献标识码:B 文章编号: 1001-2230(2015 )04-0039-04 Two-dimensional electrophoresis and its application in the study of Lactic acid bacteria 2 1 WANG Chao\ QU Xiao-jun , CUI Yan-hua ( 1. School ofFood Science and Engineering, Harbin Institute ofTechnology, Harbin 150090, China;2. Institute ofMi- crobiology, Heilongjiang Academy ofSciences , Harbin 150010, China) Abstract: Two-dimensional electrophoresis technology is one of the most popular proteornics techniques , which is the best method for large-scale screening of protein expression. The technology is combined with isoelectric focusing and SDS, achieving the separation through isoelectric point and molecular weight of proteins. Because of its convenient, fast , good repetition rate and resolution , it becomes the most widely used proteornics technique. In this paper , its technical production , principles , factors and its application in the study oflactic acid bacteria were reviewed. Key words: two-dimensional electrophoresis; isoelectric focusing; SDS; 自úluencing factors; application l3