司-反应工程.ppt

Laboratory of Industrial Catalysis Metabolic Engineering(LICME) Laboratory of Industrial Catalysis Metabolic Engineering(LICME) 自2012年6月20日起，“嘉吉烯王生物工程（武汉）有限公司”更名为”嘉必优生物工程（武汉）有限公司”. * 自2012年6月20日起，“嘉吉烯王生物工程（武汉）有限公司”更名为”嘉必优生物工程（武汉）有限公司”. * 微生物体内合成 ARA 的代谢途径大体如下：（1）在胞液中葡萄糖通过糖酵解途径生成丙酮酸；（2）丙酮酸进入线粒体后经丙酮酸脱氢酶复合体的作用生成乙酰CoA，乙酰CoA 与草酰乙酸在柠檬酸合成酶的催化作用下合成柠檬酸；（3）一部分柠檬酸进入三羧酸循环体系产生能量；另一部分柠檬酸则通过三羧酸运载体系运出线粒体膜后经柠檬酸裂解酶作用裂解为草酰乙酸和乙酰CoA，草酰乙酸再经过一系列反应生成丙酮酸，在此过程中产生的NADPH用于脂肪酸的合成；（4）乙酰CoA 在胞液中合成软脂酸；（5）软脂酸在微粒体进行碳链的延长及脱饱和作用，最终生成ARA。 * 姓名：司梦 学号：642071005020 Mass transfer and hydrodynamic characteristics of unbaffled stirred bio-reactors: Influence of impeller design 目 录 Introduction Experimental ResultsDiacussion Conclusions Introduction Nomenclature a gas–liquid interfacial area per unit volume of dis-persion [m-1] kL oxygen mass transfer coefficient [m s-1] kLa volumetric mass transfer coefficient [s-1] t time [s] t0 initial time tp oxygen probe time lag [s]liquid initial oxygen concentration in the liquid phase[kmol m-3] CL instantaneous oxygen concentration in the liquidphase [kmol m-3] N rotational speed [rpm] Ncrit critical rotational speed [rpm] Np power number [?] P power input [W] 目 录 Introduction Introduction impeller Introduction Unbaffled stirred tanks are recognized as a viable alternative to common baffled tanks for a range of processes where the presence of baffles is undesirable for some reason. e.g. for crystallizers where the presence of baffles may promote particle attrition（颗粒磨损）. In the case of shear sensitive cell cultivation (e.g. human cells), unbaffled tanks have been found to be able to provide sufficient mass transfer through the free surface vortex. In three-phase mixing operations, the blockage of gas spargers（气体喷射器） by solid particles is a common issue, which can be avoided using an unbaffled reactor operating in aerated conditions. As a consequence the need for bubble formatio