植物蛋白替代鱼粉在曼氏无针乌贼配合饲料中的应用分析.pdfVIP

蛋白饲料，对玉米蛋白粉离体消化率最低。 试验三：用膨化豆粕分别替代饲料中鱼粉蛋白质量的0%、5%、10%、15%、20%和 25% ，配制6 种等氮等能试验饲料（分别记为D 0，D 1，D 2 ，D 3，D 4 ，D 5 ）对体重 为1.34 ±0.03g 曼氏无针乌贼进行为期53d 的饲养试验，研究膨化豆粕替代鱼粉蛋白对曼 氏无针乌贼生长性能和肝脏生化指标的影响。结果表明：膨化豆粕替代鱼粉蛋白质量低 于15%时对曼氏无针乌贼生长性能、成活率和胃蛋白酶活力无显著影响（P0.05 ）。与对 照组相比，当鱼粉蛋白替代水平达15%时，成活率显著降低（P0.05 ）；替代水平达20% 时，增重率显著下降（P0.05 ）。膨化豆粕替代鱼粉对曼氏无针乌贼肌肉的粗脂肪、粗灰 分含量及脏体比和肥满度没有显著影响 （P0.05 ），但当替代水平高于15%时，肌肉粗蛋 白含量显著降低（P0.05 ）。当膨化豆粕替代鱼粉蛋白水平高于10%时，随豆粕替代鱼粉 蛋白水平的增加，胃蛋白酶活性、肝脏谷丙转氨酶（GPT ）、碱性磷酸酶（AKP ）、过氧化 氢酶（CAT ）、总超氧化物歧化酶（T-SOD）、谷草转氨酶（GOT ）、酸性磷酸酶 （ACP ） 活力先后显著降低（P0.05 ）。综合上述结果认为，在本试验条件下曼氏无针乌贼配合饲 料中膨化豆粕替代鱼粉蛋白的适宜比例为10%。 关键词 曼氏无针乌贼，消化酶，离体消化率，膨化豆粕，生长性能，生化指标 II Study on replacement of fish meal by plant protein in practical diet for Sepiella maindroni Abstract This study was conducted to evaluate the potential for use of plant protein in practical diet for Sepiella maindroni. Firstly, the effect of temperatures and pH value on activities of protease and amylase in stomach, pancreas, gastric cecum and intestine of cuttlefish Sepiella maindronie were studied by means of enzyme analyses. Then, the nutrient digestibility of seven protein feed ingredients fish meal (FM), soybean meal (SM), expanded soybean meal (ESM), fermented soybean meal (FSM), peanut meal (PM), rapeseed meal (RM) and corn gluten meal (CGM) were determined using in vitro methods for Sepiella maindronie. Finally,based on digestibility results of these protein feed ingredients, six isonitrogenous and isocaloric experimental diets were formulated to evaluate the effects of replacing ESM with FM on growth performance, pepsin activity and liver biochemical indices of Sepiella maindroni. The results were as follows: Experiment 1: The effects of temperatures