槲皮素对ApoE--小鼠动脉粥样硬化形成及 SDF-1CXCR4表达的影响分析.pdfVIP

与模型对照组比较，细胞着色较浅，表达量均有减少（P0.05 ）；与正常对 照组比较，低剂量组、中剂量组有统计学差异（P0.05 ）。不同时间点比较， SDF-1 在模型对照组的表达量随时间延长而增加（P0.05 ）；（3 ）ELISA 检 测血清中SDF-1 和CXCR4 水平，Real-time PCR 检测SDF-1 和CXCR4 的 mRNA 基因表达结果：在相同时间点比较，SDF-1 及CXCR4 在模型对照 组中表达最高，正常对照组表达最低，槲皮素组介于两者之间，且随着药 物剂量的增加SDF-1 和CXCR4 表达量进行性下降，尤其是CXCR4 在高 剂量组与正常对照组比较无统计学差异（P0.05 ）；不同时间点相同组比较， 模型对照组的SDF-1 和CXCR4 均随时间延长而增加(P0.05)，槲皮素组和 正常对照组无显著的时间依赖性。结论： 1、用高脂饮食成功在ApoE-/- 小鼠建立起动脉粥样硬化动物模型。2 、SDF-1/CXCR4 可能促进血管平滑 肌细胞增殖以及迁移至内膜，从而使内膜增厚和泡沫细胞形成，导致粥样 斑块形成。3、槲皮素可能通过抑制SDF-1 与 CXCR4 的表达途径抑制 ApoE-/-小鼠动脉粥样硬化形成，这可能是其抗动脉粥样硬化的机制之一。 关键词：槲皮素；动脉粥样硬化；SDF-1(基质细胞衍生因子-1) ； CXCR4(基质细胞衍生因子受体) 2 Research the effect of quercetin on atherosclerosis in ApoE-/- mice and expression of SDF-1/CXCR4 Abstract: Objective: To explore quercetin on ApoE - / - mice atherosclerosis formation and expression of SDF-1/CXCR4 effect. Methods: 1. Grouping of experimental animals: Forty 6-week-old ApoE-/- mice were divided randomly into four groups after one week normal feeding (N = 10): model group (high-fat diet) and group with low-dose quercetin (high-fat diet + quercetin 50 mg/kg/d, group with mid-dose quercetin (high-fat diet + quercetin 100 mg/kg/d, group with high-dose quercetin (high-fat diet + quercetin 150 mg/kg/d. At the same time, ten 6-week-old healthy C57BL/6J male mice with same genetic background as normal control group (common diet); 5 mice were randomly are sacrificed at the time of 10th week and 20th week after administration of the drugs respectively; 2. The level of SDF-1 and CXCR4 in serum were measured by Elisa assays. The atherosclerotic lesions of aortic arch were determined by HE staining and the expression of SDF-1 and CXCR4 in aorta were detected by immunohistochemical technique and Real-time PCR. Results: 1. In normal control group: at 10th week and 20th week there was no intimal hyperplasia, internal elastic l