分离纯化及单糖组成分析.PDFVIP

※基础研究 食品科学 2011, Vol. 32, No. 01 19 陈红漫， 杨佳颖，阚国仕， 任大明 (沈阳农业大学生物科技学院，辽宁 沈阳 110161) 苦瓜粗多糖(MCP)经DEAE- 纤维素凝胶柱层析及 Sephadex G-100 凝胶柱层析纯化后，得到多糖组分MCP Ia ， 经紫外光谱分析及醋酸纤维素薄膜电泳法纯度检验，结果表明 MCP Ia 是单一组分。HPLC 分析其单糖组成为L - 鼠 李糖、D - 木糖、D - 果糖和D - 半乳糖，单糖物质的量比为4.4:2.3:1:5.7 ，另外，MCP Ia 还可能含有葡萄糖；MCP Ia 红外光谱表明：苦瓜多糖存在分子内和分子间的氢键，分子中存在 C — H 键伸缩振动，存在— OH 的变形振动， 存在呋喃糖苷吸收峰，是典型的多糖类化合物。 苦瓜多糖；纯化；红外光谱 Isolation, Purification and Composition Analysis of an Antioxidant Polysaccharide Fraction from Momordica charantial CHEN Hong-man ，YANG Jia-ying ，KAN Guo-shi ，REN Da-ming (College of Biotechnology, Shengyang Agricultural University, Shenyang 110161, China) Abstract ：The crude polysaccharide (MCP) extracted from the fruits of Momordica charantial was subjected to DEAE-cellulose column chromatographic separation and Sephadex G-100 column chromatographic fractionation and a polysaccharide fraction with a high ability to scavenge superoxide anion and hydroxyl free radicals, named MCP Ia, was obtained. According to UV spectro- scopic analysis and cellulose acetate membrane electrophoresis, MCP Ia was a single composition. Its monosaccharide composition was determined by HPLC to consist of L-rhamnose, D-xylose, D-fructose and D-galactose, in a molar ratio of 4.4:2.3:1:5.7, with the possibility of containing glucose. The IR spectral analysis showed that there were intramolecular hydrogen bond and intermolecular hydrogen bond in MCP Ia. In addition, the existences of stretching vibration of the carbon-hydrogen and deformation vibration of hydroxyl group and characteristic absorption of ribofuranoside were also demonstrated. Therefore, MCP Ia is a typical polysaccharide. Key words ：Momordica charantial polysaccharides ；purifi