基于COI序列的DNA条形码在中国沿海缀锦蛤.PDFVIP

动 物 学 研 究 2010，Aug. 31(4): 345−352 CN 53-1040/Q ISSN 0254-5853 Zoological Research DOI ：10.3724/SP.J.1141.2010.04345 基于 COI 序列的 DNA 条形码在中国沿海缀锦蛤 亚科贝类中的应用分析 * 陈 军，李 琪 ，孔令锋，郑小东，于瑞海 ( 中国海洋大学 海水养殖教育部重点实验室，山东 青岛 266003) 摘要：该研究探讨了将 COI 序列应用于中国沿海缀锦蛤亚科贝类物种鉴定的可行性，获得了该亚科 5 属 11 种贝类51 个个体的43 个单倍型序列。碱基替换饱和性分析表明，颠换未出现饱和现象，而转换在序列分化达到 10%至15%时即到达饱和。单倍型Hap33 可能是由杂交引起的，排除此单倍型，种内个体间遗传距离在0%到2.02% 之间，平均为 0.46%，属内不同种个体间遗传距离在 17.21%～32.24%之间，平均为 24.96% ，存在条形码间隙； 11 种缀锦蛤亚科贝类在邻接树和贝叶斯树上都独立的单系群。该研究表明，基于COI 的DNA 条形码技术能够将 研究所涉及的约98%的缀锦蛤亚科贝类鉴定到种的水平，因此，利用DNA 条形码技术可以对缀锦蛤亚科贝类进 行有效地分类鉴定。 关键词：缀锦蛤亚科; DNA 条形码; COI 序列; 物种鉴定 中图分类号：Q959.215; Q3491; Q951.3 文献标志码：A 文章编号：0254-5853-(2010)04-0345-08 COI-based DNA Barcoding in Tapetinae Species (Mollusca, Bivalvia, Veneridae) Along the Coast of CHINA * CHEN Jun, LI Qi , KONG Ling-Feng, ZHENG Xiao-Dong, YU Rui-Hai (Fisheries College, Ocean University of China, Qingdao 266003, China) Abstract: DNA barcoding has exhibited charming effectiveness in species diagnosis, but some studies suggested the proportion of taxa that cannot be barcode-distinguished was still high. In the present study, the efficiency of the DNA barcoding for delimiting species of subfamily Tapetinae along the coast of China was tested. Fifty one original COI sequences of 11 species in five genera were analyzed. Among these sequences, 43 haplotypes were identified. Saturation plots generated for DNA barcode revealed that transitions became saturated