呼吸道合胞病毒感染对豚鼠咳嗽相关气道功能及其神经递质的.PDFVIP

182 ( ) 2011 6 5 3 Chin J Asthm a ( Electronic E ition) , June 2011, Vol. 5, No. 3 叶新民 钟南山 刘春丽 陈如冲 赵谨 RSV( ) , SPF 60 , , 61228 42 , 10 RSVBuxco ( CRS) ( AR) PCR ( Realtime PCR) 1(VR1) mRNA , VR1 9. 5( PGP9. 5) 6122842 CRS[ ( 8. 00 3. 86) ( 8. 70 6. 20) (7. 60 4. 40) ( 6. 70 3.71) CCnt] [ (2. 50 1.43) CCnt ] ( P 0. 05) , 12 ( P 0. 01) 12 AR 2 ( 1 069 156) % ( 1 846 285) % , ( P 0. 050. 01) 612 28 VR1 mRNA 1. 57 0. 431. 61 0. 47 1. 68 0. 56, ( 1.00 0. 34) ( P 0.05) , 28 , 28 VR1 ( P 0. 05) ; 12 PGP9. 5( P 0. 05) CRS AR RSV , VR1 PGP9. 5 ; ; ; ; 9. 5 Effect s of respiratory syncytial virus infecti on on coughrelated ai rway f uncti on and neurotransmitter in guinea pi gs YE X inmin, ZH ON G N anshan, L I U Chunli, CH EN Ruchong , ZH A O J in. S tat K y L aboratory of R sp iratory D is as s , th First Af f iliat d H osp ital of Guangz hou M dical Coll g , Guang z hou I ns titut of R sp irato ry D is as , Guang z hou 510120 , China Corr sp onding author : L I U Chunl i, Email: chunli. liu@ y ahoo . com. cn Abst ract Objective To investigate the effect s of respiratory syncyt ial virus ( RSV) infect ion on coughrelate airw ay function an neurot ransm itter in guinea pigs an t o explore t he pat hogenesis of cough after RSV infection. Methods Sixty SPF male guinea pigs were ran omly ivi e into control group, asthma group an four RSVinfecte groups ( 6, 12, 28 an 42 group) , ten in each group. Infecte animals w ere inoculate by intranasal instillation of RSV suspension. Plethysmography w as use to assess cough ref lex sensitivity ( CRS) an airw ay responsiveness (AR) . Vanilloi receptor1 ( VR1) mRNA level was etect e by realtime PCR. The p