油松花粉蛋白提取工艺的研究_范三红_王相帅_王亚云_胡雅喃_刘晓华.pdfVIP

DOI ：CNKI:11-1759/TS1503.059 网络出版时间：2013-07-09 15:03 网络出版地址：/kcms/detail/11.1759.TS1503.059.html 油松花粉蛋白提取工艺的研究 范三红，王相帅，王亚云，胡雅喃，刘晓华 （山西大学生命科学学院，山西太原 030006 ） 摘要：为了充分利用油松花粉中的蛋白质，对油松花粉的基本组分进行测定，采用碱提酸沉法提取油松花 粉中的蛋白，通过正交实验确定出油松花粉蛋白的最佳提取条件。结果表明，油松花粉中水分含量、灰分 含量、蛋白质含量、脂肪含量、还原糖含量分别为8.72%、3.19%、20.32% 、2.84% 、24.71% 。油松花粉蛋 白的等电点pI3.6 ，在此基础上研究油松花粉在料液比、提取时间、提取温度、提取pH对油松花粉蛋白提取 率的影响。经过单因素实验筛选较优水平后，设计正交实验，采用极差分析和方差分析，得出油松花粉蛋 白的最佳提取条件为料液比为1:40 （g/mL ）；提取pH为11;提取时间为80min；提取温度为55℃；在此提取 条件下，油松花粉蛋白的提取率为56.3%。 关键词：油松花粉，蛋白质，提取，正交试验 Optimization of extraction conditions for protein from pine pollen FAN San-hong, WANG Xiang-shuai,WANG Ya-yun, HU Ya-nan, LIU Xiao-hua, （College of Life Science, Shanxi University, Taiyuan 030006, China ） Abstract: In order to make full use of protein in the pollen, and to determine the basic set of pine pollen, I used the alkali extraction of pine pollen in protein extraction and acid precipitation, and the orthogonal experiment to determine the best extraction conditions of pine pollen protein solution. Barley malt roots contained 20.32% protein, 3.19% ash, 8.72% water, 2.84% fat and 24.71% reducing sugar content. Pollen protein isoelectric point of pI is 3.6, on this basis to research in solid-liquid ratio, alkali extraction time, alkali extraction temperature, alkali extraction rate for the effect of pH on pollen protein extraction. Through single factor experiment to select the optimum level, to design orthogonal experiment, to apply the range analysis and v