A comparative stud的y on fungi Cordyceps militaris and Cordyceps sinensis submerged mycelial cultures.pdfVIP

A comparative stud的y on fungi Cordyceps militaris and Cordyceps sinensis submerged mycelial cultures.pdf

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A comparative stud的y on fungi Cordyceps militaris and Cordyceps sinensis submerged mycelial cultures

Journal of Applied Microbiology 2005, 99, 728–738 doi:10.1111/j.1365-2672.2005.02682.x A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures H.O. Kim and J.W. Yun Department of Biotechnology, Daegu University, Kyungbuk, Korea 2004/1492: received 22 December 2004, revised 9 May 2005 and accepted 10 May 2005 ABSTRACT H . O . K I M A N D J. W . Y U N . 2005. Aims: The present study comparatively investigates the optimal culture conditions for the production of exopolysaccharides (EPS) and cordycepin during submerged mycelial culture of two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis. Methods and Results: Fermentations were performed in flasks and in 5-l stirred-tank fermenters. In the case of C. militaris, the highest mycelial biomass (22Æ9 g l)1) and EPS production (5 g l)1) were achieved in a medium of 40 g l)1 sucrose, 5 g l)1 corn steep powder at 30C, and an initial pH 8Æ0. The optimum culture conditions for C. sinensis was shown to be (in g l)1) 20 sucrose, 25 corn steep powder, 0Æ78 CaCl , 1Æ73 2 MgSO Æ7H O at 20C, and an initial pH 4Æ0, where the maximum mycelial biomass and EPS were 20Æ9 and 4Æ1 g l)1 4 2 respectively. Cordycepin, another bioactive metabolite, was excreted at low levels during the early fermentation period (maximum 38Æ8 mg l)1 in C. militaris; 18Æ2 mg l)1 in C. sinensis). Conclusions: The two fungi showed different nutritional and environmental requirements in their submerged cultures. Overall, the concentrations of mycelial biomass, EPS and cordycepin achieved in submerged culture of C. militaris were higher than those of C. sinensis. Significance and Impact of the Study:

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