炎症性肠病基因共表达网络构建及分析.docVIP

炎症性肠病基因共表达网络构建及分析 　　[摘要] 目的 用共表?_网络的系统生物学分析炎症性肠病（Inflammatory Bowel Disease，IBD）主要包括溃疡性结肠炎（Ulcerative colitis，UC）和克罗恩病（Crohns disease，CD）的生物学功能，为疾病机制研究和药物开发提供重要信息。 方法 本文以公共数据库中的212个IBD RNA-Seq数据为对象，用权重基因共表达分析（Weighted Gene Co-expression Network Analysis，WGCNA）方法构建其基因共表达网络，数据库注释、可视化和综合发现（Database for Annotation，Visualization and Integrated Discovery，DAVID）工具分析模块数据，并利用药物处理基因表达谱数据库（Connectivity map，CMAP），对可能的治疗药物进行筛选。 结果 （1）共鉴定出12个具有生物学意义的共表达基因模块，模块的功能与免疫应答、血管生成、转录、翻译、能量代谢等生物学过程相关，并挖掘出每个模块的关键节点基因；（2）发现UC和CD间在胺基糖代谢、O-Glycan合成、血管生成、B细胞受体信号通路上有显著差异；（3）挖掘出12种潜在治疗药物。 结论 本文首次鉴定出IBD基因功能模块，这些模块可能代表疾病的主要特征，为疾病机制研究和药物开发提供重要理论依据 [关键词] 炎症性肠病；基因共表达网络；溃疡性结肠炎；克罗恩病 [中图分类号] R574.1；Q933 [文献标识码] A [文章编号] 1673-9701（2017）09-0035-04 Construction and analysis of gene co-expression network of inflammatory bowel disease YE Hua1 LIU Huiwei2 HUANG Shiliang1 ZHANG Xuesong1 HUANG Sha1 GAO Zhiqiang1 LIU Wei3 SONG Yufei1 1.Department of Gastroenterology， Ningbo University Affiliated Ningbo Medical Center Li Huili Hospital， Ningbo 315040，China； 2.Ningbo University School of Medicine， Ningbo 315211， China； 3.School of Life Science， Fujian Agriculture and Forestry University， Fuzhou 350002， China [Abstract] Objective To analyze inflammatory bowel disease（IBD） including Ulcerative colitis（UC） and Crohn’s disease （CD） by using the systematic biology of co-expression networks，and to provide important information for disease mechanism research and drug development. Methods A total of 212 IBD RNA-Seq data in the public database were chosen as the study subjects. Construction of gene co-expression network was carried out by weighted gene co-expression network analysis （WGCNA） and the module data was analyzed by database for annotation， visualization and comprehensive discovery.The possible therapeutic drugs were screened using the drug-treated connectivity map （CMAP）. Results （1） A total of 12 biologically significant cofactor gene modules were identified. The function of the module was rela