大鼠出生后内侧膝状体腹侧部神经元电生理与形态学特性的发育变化.pdf

Acta Physiologica Sinica, June 25, 2005, 57 (3): 333-339 333 * 400038 (biocytin) (postnatal day, P) 3~30 (P3~30)(ventral partition of medial geniculate body, MGBv) (1) P3~30 MGBv −40 mV −67 mV (P0.01); 1 832 M Ω 806 M Ω (P0.01); 2.55 ms 0.96 ms (P0.01) (P0.01); (2)K+ 4-AP P6 MGBv P16 Ca2+ CdCl2 P16 MGBv (3)biocytin MGBv MGBv (tufted neuron) MGB v Q426; Q424; R322.81; R338.8 Changes in electrophysiological and morphological properties of neuron in the ventral partition of medial geniculate body during the postnatal development of rats YAO Xiao-Hong, XIONG Ying* Department of Physiology, Third Military Medical University, Chongqing 400038 China Abstract: We investigated the electrophysiological and morphological characteristics of the neurons in the ventral partition of medial geniculate body (MGBv) in the rat during postnatal development (postnatal day 3~30, P3~30) with whole-cell patch clamp recording and intracellular labeling technique. The results are as follows: (1) There was an increase in the negativity of the resting membrane potential, a decrease in the input resistance and the time constant of the MGBv neurons of rats during the postnatal development; the amplitude, threshold and duration of the action potentials were also different on different postnatal days; (2) During the postnatal development, the changes in voltage-dependent ion channels dramatically influenced the waveform of action potential in the MGBv + neurons. Application of 4-AP, a K -channel blocker, reduced the amplitude and prolonged the durations of the action potentials in P6 2+ neurons, and inactivated the neurons at the end of the pulse in P16 rats. Application of