期刊Fractionation of high-dose electron beam irradiation of BPTB grafts provides significantly improved viscoelastic and structural properties compared to standard gamma irradiation.pdfVIP

Knee Surg Sports Traumatol Arthrosc (2011) 19:1955–1961 DOI 10.1007/s00167-011-1518-9 EXPERIMENTAL STUDY Fractionation of high-dose electron beam irradiation of BPTB grafts provides significantly improved viscoelastic and structural properties compared to standard gamma irradiation A. Hoburg • S. Keshlaf • T. Schmidt • M. Smith • U. Gohs • C. Perka • A. Pruss • S. Scheffler Received: 2 December 2010 / Accepted: 12 April 2011 / Published online: 4 May 2011 Springer-Verlag 2011 Abstract Results Fractionation of ebeam irradiation resulted in Purpose Irradiation [30 kGy is required to achieve ste- significantly higher failure loads (1,327 ± 305) than with rility against bacterial and viral pathogens in ACL allograft one-time ebeam irradiation (1,024 ± 204; P = 0.008). sterilization. However, doses [20 kGy substantially reduce Compared to gamma irradiation, significantly lower strain the structural properties of soft-tissue grafts. Fractionation (2.9 ± 1.5 vs. 4.6 ± 2.0; P = 0.008) and smaller cyclic of irradiation doses is a standard procedure in oncology to elongation response (0.3 ± 0.2 vs. 0.6 ± 0.4; P = 0.05), reduce tissue damage but has not been applied in tissue as well as higher failure loads (1,327 ± 305 vs. graft sterilization. 827 ± 209; P = 0.001), were found. Compared to non- Methods Forty-four human 10-mm wide bone-patellar- irradiated BPTB grafts, no significant differences were tendon-bone grafts were randomized into four groups of found for any of the biomechanical parameters. Non-irra- sterilization with (1) 34 kGy of ebeam (2) 34 kGy gamma diated control