Survivin基因siRNA对胃癌细胞生长的抑制作用.pdfVIP

2007年l2月第22卷第 l2期 Chin J Gen Surg．December 2Oo7．v01．22．No．12 · 925· ． 论著 Survivin基因siRNA对胃癌细胞 生长的抑制作用 贾绍昌 苏长青 张卫兵 王跃华 俞永忠 【摘要】 目的 构建携带沉寂Survivin基因的特异性siRNA的表达质粒，鉴定其在胃癌细胞内 对Survivin基因表达的沉寂以及对肿瘤细胞生长的抑制。 方法 合成Survivin基因特异性siRNA， 插入到表达载体pAdGFP中构建成pAdGFP—siRNA。培养人胃癌细胞SGC-7901，转染pAdGFP—siRNA， 研究该载体在人胃癌细胞内对Survivin基因表达的抑制作用以及对肿瘤细胞生长的抑制作用。 结果 转染pAdGFP—siRNA后，胃癌细胞SGC-7901的生长受到明显抑制，抑制率为68．2％。免疫组 化检查显示，特异siRNA显著降低了癌细胞Survivin基因的表达。 结论 siRNA技术能够沉寂 Survivin基因的表达，抑制癌细胞的生长，改善治疗效果。 【关键词】 胃肿瘤；基因；遗传载体；转染 Effects of survivin gene siRNA on the growth of g~tHc cancer cell line JIA Shao—chang ． SU Chang—qing，z ⅣG耽 —bing，WANG Yue—hurt，YU Yong—zhong． Department of Surgical Oncology， Na ng 81 Ho~ital ofthe Chinese People s Liberation Army，Na ng 210002，China Corresponding author：SU Chang—qing，E—mail：Changqing@EHBH．cn 【Abstract】 Objective To construct an expression plasmid carrying the specific siRNA of survivin gene．and to evaluate its silencing effect on the expression of survivin geBe and its inhibition effect on the growth of gastric cancer cells． Methods The specific siRNA of survivin gene Was designed and synthesized．and an expression plasmid pAdGFP—siRNA Was constructed．Gastric cancer cell line SGC-7901 Was cultured and transferred with pAdGFP—siRNA，then the silencing of survivin gene expression and the growth inhibition of cancer cell mediated by pAdGFP—siRNA were identified． Results The growth of gastric cancer cells Was inhibited after tmns~rring the pAdGFP—siRNA．with the inhibition rate of 68．2％ compared to the control group．Immunohistochemistry showed that the specific siRNA markedly silenced the expression of survivin gene in cancer cells． Conclusions The overexpression of survivin gene in gastric cancer cells results in the high proliferation and the resistance to the chemo—and radio—therapy of the cancer cells．The sp