火焰原子吸收光谱法测定人体皮屑中钠、钾含量及 - 第三军医大学学报.docVIP

肥胖患者皮肤组织钠钾离子浓度的检测及临床意义 宋媛媛，王 非，李 强，王利娟，闫振成，倪银星，何洪波，杨建江，祝之明 （400042 重庆，第三军医大学大坪医院野战外科研究所：全军高血压代谢病中心，重庆大坪医院高血压内分泌科，重庆市高血压研究所） [摘要] 目的 测定肥胖患者皮屑中钠、钾离子浓度的含量及皮肤组织钠、钾转运相关蛋白的表达，并探讨其临床意义。方法 招募127例重庆地区自愿者，并以体质指数（BMI）和腰围（WC）值，将其分为肥胖、超重、健康组和腹型肥胖、健康组，刮取研究对象双前臂内侧皮屑，用消化液（1.5 mL×65％硝酸和0.5 mL×70％高氯酸）加热溶解后，使用火焰原子分光光度计检测溶液中钠、钾的含量；提取小鼠及人皮肤组织蛋白，用蛋白印迹法检测糖皮质激素受体、醛固酮受体、Na+-K+-ATP酶表达。结果 在最适反应条件下，本方法的相对标准偏差在0.34～1.45，回收率为93.1% ～ 107.2%；肥胖组与健康组相比，皮屑钠、钾值均有显著性差异（P=0.002、P=0.000），肥胖组与超重组比差异也有显著性（P=0.002、P=0.000），超重组与健康组比较均无差异。Western blot检测小鼠和人皮肤组织中糖皮质激素受体、醛固酮受体、Na+-K+-ATP酶3种蛋白均有表达。结论 建立检测人体皮屑中钠、钾离子浓度的方法，肥胖者皮屑钠、钾含量明显升高，提示本方法可简易地反映临床肥胖患者组织电解质变化，钠、钾转运相关的3种蛋白在小鼠和人皮肤组织中均有表达。 [关键词] 火焰原子吸收光谱法，原子分光光度计，皮肤，钠，钾 [中图法分类号] [文献标志码] A Skin contents of sodium and potassium and clinical significance in obesity Song Yuanyuan, Wang Fei， Li Qiang, Wang Lijuan,Yan Zhencheng, Ni Yinxing, He Hongbo, Yang Jianjiang, Zhu Zhiming （Center for Hypertension and Metabolic Diseases, Department of Hypertension and Endocrinology, Chongqing Institute of Hypertension,Daping Hospital, Third Military Medical University, Chongqing, 400042, China） [Abstract] Objective To determine the concentration of sodium and potassium in the dander from obese patients, and the expression of sodium- and potassium-related transporters at skin tissue, and to explore their clinical significance. Methods A total of 127 volunteers, 75 females and 52 male with an average age of 52.83?.11 (ranging from 40 to 60) were trecruited from Chongqing region. The volunteers were divided into normal weight (n=50), overweight (n=56) and obese group (n=21) by their body mass index (BMI), or non-abdominal obesity (n=81) and abdominal obesity group (n=46) by their waist circumference (WC). Dander scraped from medial forearm of subjects was digested by heated solution (1.5 ml 65% nitric acid and 0.5ml 70% perchloric acid), then the concentration of sodium and potassium in the solution was detected by flame atomic spectrophotometry. Glu