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植物组织造就(国外英语资料)
植物组织培养
1 washing. The culture bottles and other containers should be soaked in water, then brush the dirt inside the bottle, and then washing powder, washing and washing clean. Washing utensils should not be hung with water, the inside and outside wall water film uniform, placed in the oven drying. New glass purchased more or less containing free alkaline substances. Soak overnight with 1% hydrochloric acid before use, then rinse with clean water. Rinse the distilled water over and dry it away. Glassware contaminated must be in 30 minutes to 121 DEG C high pressure steam sterilization, pour out the residue with the hair brush to the wall of the culture medium and the plaque, and then rinse with water, distilled water rinse again to dry. Remember not to flush with water directly, otherwise environmental pollution will be completed.
2 prepare mother liquor and hormone. According to the MS medium formula given by the data, the mother liquid of a to D is prepared, and the mother liquor is divided into 4 solution bottles. The mother liquor is labeled separately, indicating the mother liquor name, configuration, endorsement and date. Storage time is not too long, inorganic salt, wood is best to use up in a month, when the mother liquor in mold or precipitation, the mother liquor can not be used. Note that iron salts are to be individually blended. Dissolve 2,4-D, 6-BA with a small amount of alcohol, and then dilute with water to the desired concentration.
3 preparation medium. The mother liquid is taken out in proportion, and the corresponding volume is injected into the beaker in sequence. Adding sucrose, dissolving sugar and adding water to 1L. Use the pH meter to adjust the pH value to 5.8. Adjust the hydrochloric acid and sodium hydroxide with reagent 1mol/L. Add the agar and heat it up, then mix it while heating, so as not to make the paste bottom.
4 packing sterilization. The prepared medium shall be packed in a tapered flask as soon as possible. The methods of pac
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