实时荧光定量PCR检测临床上的运用及意义.pdf

实时荧光定量PCR 检测临床上的运用与意义 叶明奇 （厦门安普利生物工程有限公司，福建 厦门 343，技术工程部） 【摘要】目的分析临床使用实时荧光定量 PCR 法在临床检测的各个项目情况，并探讨实时荧 光定量 PCR 法的临床意义。主要介绍核酸 DNA 或 RNA 的提取、实时荧光定量 PCR 技术以及 实时荧光定量 PCR 方法临床运用的意义：乙型肝炎病毒核酸实时荧光定量ＰＣＲ检测在临床 诊断中的应用、实时荧光 PCR 检测丙型肝炎病毒核酸及其临床应用、实时荧光定量 PCR 法 在检测血浆结核杆菌 DNA 的临床应用、实时荧光定量 PCR 检测 HPV 病毒的临床运用、泌尿 生殖道感染沙眼衣原体的临床应用、传染性疾病检测临床应用、测胰腺癌中 HIF1α 、VEGF 和 bFGF 的表达及其临床意义、绵羊肺腺瘤病毒检测、胃癌淋巴结微转移敏感且特异的方法， 实时荧光定量 PCR 法在临床检测的运用在临床分期、判断预后以及治疗方案选择具有重要意 义。 【关键词】实时荧光定量 PCR；乙型肝炎；丙型肝炎；传染性疾病；HPV；MTB； [Abstract] Objective using real-time fluorescence quantitative PCR method in clinical detection of each project, and to explore the clinical significance of real-time fluorescent quantitative PCR method.Mainly introduces the nucleic acid extraction of DNA or RNA, real-time fluorescent quantitative PCR and real-time fluorescence quantitative PCR method for clinical application significance: hepatitis B virus DNA by real-time fluorescent quantitative PCR assay application in clinical diagnosis, real-time fluorescent PCR detection of hepatitis C virus nucleic acid and its clinical application, the real-time fluorescence quantitative PCR assay in the detection of Mycobacterium tuberculosis DNA in plasma clinical application, real-time fluorescence quantitative PCR detection of HPV virus, the clinical application of urogenital Chlamydia trachomatis infection in the clinical application of infectious diseases, clinical application, measured in pancreatic cancer HIF1 α, VEGF and bFGF expression and its clinical significance, sheep pulmonary adenomatosis virus detection of lymph node micrometastasis, sensitive and specific method, real-time fluorescence quantitative PCR method applied in clinical detection in clinical staging, prognosis and treatment options have important significance. [keyword] real-time fluorescence quantitative PCR; hepatitis B; hepatitis C virus; infectious diseases; HPV; MTB; PCR法最初由Khorana(1971)等提出在体外经DNA变性,与适当引物杂交,再用DNA聚合酶 延伸