中国大耳白肉兔IFN-γ、IL-2和IL-4基因的克隆测序与进化分析.pdfVIP

第25卷 第4期 四川农业大学学报 Vol_25 No．4 2007年 l2月 Journal of Sichuan Agncultural University Dec．2007 Cloning，Sequencingand Evolution Analysis of IFN—Y， IL一2 and IL～4Gene in Chinese White Rabbit HUANG Dao-chao ，YANG C-uan8一you。 ，LAl Sons-j i82 (1．College of Veterinary Medicine，Sichuan Agricultural University； 2．College of Animal Science and Technology，Sichuan Agricultural University，Yaan 625014，Sichuan，China) Abstract：With reverse transcription polymerase chain reaction(RT—PCR)，IFN一7，IL一2 and IL 一 4 gene were gained from total RNA which was extracted from ConA-stimulated-cultured peripheral blood lymphocytes of Chinese white rabbit．The~uence analysis revealed that：①Compared with one published sequence(sequence number：AB010386)，the homology of nucleotide sequence and amino acid sequence of IFN一7gene(sequence number：DQ852341)were both 100％；compared with other mammals in Genbank，including dog，cat，human，pig，cow，horse，goat，giant panda，rat，and so on，the homology of nucleotide sequence was between 63．0％(Rat)and 77．6％(Human)，the ho— mology of amino acid sequence was between 41．7％(Rat)and 65．7％(Human)；②Compared with one published sequence(sequence number：AF068057)，the homology of nucleotide sequence and amino acid sequence of IL一2 gene(sequence number：DQ852342)were 99．6％and 100％respective— ly；compared with other mammals in Genbank，including dog，cat，human，pig，cow，horse，goat， giant panda，rat，and so on，the homology of nucleotide sequence was between 64．8％ (Rat)and 86．0％(Human)，the homology of amino acid sequence was between 55．7％(Rat)and 80．1％(Hu— man)；⑧Compared with one published sequence(sequence number：AF169169)，the homology of nu— cleotide sequence and amino acid sequence of IL一4 gene(sequence number：DQ852343)were both 100％；compared with other mammals in Genbank，includ