凝血酶预处理减轻凝血酶所致的脑水肿.pdfVIP

中南大学学报(医学版) _， 眦sDm^ ( ＆ ) 2007，32(6) lO93 凝血酶预处理减轻凝血酶所致的脑水肿 刘劲芳 ，刘运生 ，方加胜 ，史保中 ，徐立新 (1，中南大学湘雅医院神经外科，长沙410008；2，河南科技大学第一附属医院神经外科， 河南 洛阳471003；3，常德市第一人民医院神经外科，湖南 常德415003) [摘要] 目的：探讨凝血酶预处理对脑水肿的影响，检测肿瘤坏死因子一 (tumor necrosis factor— ， TNF— )在脑组织内的表达并分析其与凝血酶所致脑水肿的关系。方法：建立大脑皮质内血肿模型。选 择健康成年sD大鼠40只，随机分为作为对照的未预处理组(sT组)和小剂量凝血酶(1 u)预处理组 (rrr组)。观察不同时相脑组织含水量及钠含量的变化及TNF— 在脑组织内的表达。结果：sT组脑组 织含水量和钠含量明显高于rrr组，且第l天高于第3天。rrr组与sT组脑组织内TNF— 阳性表达与含 水量的变化相一致。结论：凝血酶预处理可以减轻凝血酶所致的脑水肿；TNF— 的表达与凝血酶所致的 脑水肿有关。 [关键词] 脑水肿； 凝血酶； 凝血酶预处理； 肿瘤坏死因子一 ； 鼠 [中图分类号] R651-l5 [文献标识码] A [文章编号] l672—7347(2007)06一l093—05 Thrombin preconditioning reduces brain injury caused by intracerebral infusion of high dose thrombin LIU Jin．fang ，LIU Yun．sheng ，FANG Jia．sheng ，SHI Bao．zhong ，XU Li．xin (1，Department of Neurosurgery，Xiangya Hospital，Central South University，Changsha 410008；2，Department of Neurosurgery，First Affiliated Hospital，Henan University of Science and Technology，Luoyang Henan 471003； 3，Department of Neurosurgery，First People’S Hospital of Changde，Changde Hunan 41 5003，China) Abstract： Objective To explore the effect of the cerebral thrombin preconditioning on the thrombin-induced brain edema，to detect the expression of tumor necrosis factor-o【(TNF-o【)，and to analyse the relationship between TNF-o【and the thrombin-induced brain edema．Methods Forty SD rats were randomly divided into a ST group and a TY group．The rats received 5 0 L saline(ST group)or 1 U thrombin infusion(TY group)，and received the second infusion(1 0 U thrombin)24 h later．The rats were sacrificed at 24 and 72 h after the second infusion in order to examine the changes of