半夏生物总碱和钩藤生物总碱抗小鼠、大鼠惊厥的协同作用.pdfVIP

Journal 01 Chinese Pharmaceutical Sciences 139 Synergistic effect of pine蓝a total alkaloids and uncaria total alkaloids on an往convulsant ac柱。n in mice and rats Y血-x臼 Cher毡， Ming-Zheng Wang* , J血g-Jing Chen, Rong Yang, Xin-Gu He , Yong-Gang ~毡， Li-Hua Yang and Ming-Sheng Zhang Dψar栩栩tofPhw宵的cology， Shanxi Medical Universl纱，岛~vuan 030001, China Abstract: Aim To inves位gate the s严ergistic effect of the ∞mbination of pinellia total alkaloidσTA) and uncariaωtal alkaloid (UTA), and explore the mechanism of anticonvuIsant action. Methods Anticonvulsant and toxic effect profiles of combinations ofPTA with UTA, alone and at three fixed ratios of 1:4, 1:1 ,4:1 ,were evaluated in maximal electroshock (MES)-induced se泣ures and acute toxicity test in mice. Respective ED 阻d LD50 were calculated with Bliss s method. The泣 syne怒istic effect were evaluated by isobolographic 50 analysis and allowed 也e determination of benefit indices (B I) for respective combinationsτhe model of convulsive rats kindled by penicillin topìcally injected into cortex was used to investigated the content of Glu, Asp, Glyand GABA in hippocarnpus using high performance liquid chromatographyσIPLC).Re翠ultsComb妇ations ofPTAand UTAat the ratio of 4:1 were synergistic in MES test and antagonistic in acute toxicity test , showing the best pro纽e for combinations ofPTA with UτA 量1 contrast，也e ratios of 1:4 and 1: 1, d岱pi岱明le笔istic in MES test , were addi由e in acute toxicity test The 4: 1combinatìon and two drugs alone significantly decreased Glu level 姐d increased GABA level in the h羊肉campus， butthεGABA level in the 4: 1ωmbination group was higher 也扭 that in the two drugs alone groups. They did not have significant inf1uen∞ on the levels ofAsp and Gly.