循环肿瘤DNA检测细节：采血管的选择.pdf

HHS Public Access Author manuscript A Clin Biochem. Author manuscript; available in PMC 2016 October 01. u t h Published in final edited form as: o r Clin Biochem. 2015 October ; 48(15): 993–998. doi:10.1016/j.clinbiochem.2015.07.097. M a n u s c Comparison of cell stabilizing blood collection tubes for r i p t circulating plasma tumor DNA 1 1 1 1 Patricia Valda Toro , Bracha Erlanger , Julia A. Beaver , Rory L. Cochran , Dustin A. 1 1 1 1 1 VanDenBerg , Elizabeth Yakim , Karen Cravero , David Chu , Daniel J. Zabransky , Hong 1 1 1 1,2 1 Yuen Wong , Sarah Croessmann , Heather Parsons , Paula J. Hurley , Josh Lauring , and Ben Ho Park1,3 A u 1The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of t h o Medicine, Baltimore, MD r M 2The Brady Urologic Institute, Department of Urology a n u 3The Whiting School of Engineering, Department of Chemical and Biomolecular Engineering, The s c Johns Hopkins University, Baltimore, MD r i p t Abstract Objectives—Circulating plasma DNA is being increasingly used for biomedical and clinical research as a substrate for genetic testing. However, cell lysis can occur hours after venipuncture when using standard tubes for blood collection, leading to an increase in con