纳米复合树脂FiltekZ350对人牙髓细胞的毒性.docVIP

纳米复合树脂(Filtek Z350)对人牙髓细胞的毒性 作者：雷丽珊, 黄晓晶, 郑碧琼, 张 明, 钟 声, 林 昱　 作者单位：福建医科大学 附属口腔医院牙体牙髓科,福州 350002 【摘要】 目的 比较纳米复合树脂(Filtek Z350)与临床常用修复材料(Dyract-AP复合体、TPH复合树脂)的细胞毒性。 方法 原代培养人牙髓细胞(HPC),MTT比色法测定3种材料对HPC增殖的影响,扫描电镜观察HPC在材料表面生长情况。 结果 25%Filtek Z350细胞毒性为0级,小于Dyract-AP和TPH(P0.05)。扫描电镜观察HPC在3种材料表面均可贴附生长。 结论 3种牙体修复材料对HPC的生长、增殖、贴附无不良影响,均具良好的生物相容性。Filtek Z350的细胞毒性小于Dyract-AP及TPH。 【关键词】 复合树脂类； 牙髓； 细胞毒性； 比色法； 氮蓝四唑； 显微镜检查，电子，扫描 Cytotoxicity Test of Filtek Z350 Nano-Composite Resin upon Human Pulp Cell in Tissue Culture in vitro LEI Lishan, HUANG Xiaojing, ZHENG Biqiong, ZHANG Ming, ZHONG Sheng, LIN Yu School of Stomatology, Fujian Medical University, Fuzhou 350002, China ABSTRACT: Objective To compare the cytotoxicity of three kinds of dental restorative material, nano-composite resin Filtek Z350, compomer Dyract- AP and traditional composite resin TPH on cultured human pulp cell. Methods Human pulp cell were cultured by tissue culture from fresh dental pulp of teenagers, the effect of each material on viability of cultured cell was measured by MTT assay. The adherence of human pulp cell to different material surface was observed under scanning electron microscope(SEM). Results The Filtek Z350 nano-composite resin showed the least cytotoxicity among three kinds of material tested(P0.05). Under SEM observation, pulp cells showed good adherence to all three kinds of material. Conclusion All these three kinds of dental restorative material have good biocompatibility and were biologically safe. The cytotoxicity of Filtek Z350 nano-composite resin was the least among 3 materials tested. KEY WORDS: composite resins; dental pulp; cytotoxicity; colorimetry; nitroblue tetrazolium; microscopy,electron,scanning 随着纳米技术在口腔修复材料中的应用和发展,已在很大程度上改善了传统材料的性能［1-2］。Filtek Z350是一种将填料纳米化的复合树脂,经测试其耐磨性、抛光性等主要物理机械性能指标高于或相当于其他复合树脂［3］。笔者采用体外细胞培养技术和四甲基偶氮唑盐(MTT)比色法测定该材料和临床常用的两种牙体修复材料浸渍液对人牙髓细胞增殖情况的影响,扫描电镜观察人牙髓细胞在3种材料表面的生长贴附