巨噬细胞移动抑制因子与大肠癌肝转移的关系.pdfVIP

2007年11月第22卷第11期 Chin J Gen Surg。November 2007，Vo1．22．No．11 · 855· ． 论著 ． 巨噬细胞移动抑制因子与大肠癌 肝转移的关系 何兴祥 李晓宇 郝元涛 文卓夫 黄世章 苏杭 陈德 【摘要】 目的 检测巨噬细胞移动抑制因子(maerophage migration inhibitory factor，MIF)在大肠 癌组织、大肠癌肝转移灶中的表达以及大肠癌患者血清中的水平，初步分析MIF与大肠癌肝转移的 关系。方法 应用免疫组化技术，检测49例大肠癌组织及其癌旁相对正常肠组织，与1O例大肠癌 肝转移组织及转移灶旁相对正常肝组织，以及5例正常肝组织中MIF的表达。应用ELISA法测定 3O例大肠癌患者和17例健康志愿者血清MIF水平。采用Logistic回归分析大肠癌肝转移与各种临 床病理因素、血清MIF水平的关系。结果 (1)大肠癌组织中MIF的表达有肝转移者高于没有肝转 移者，大肠癌患者有肝转移者血清MIF水平也高于无肝转移者。(2)肝内大肠癌转移组织中MIF表 达阳性。(3)大肠癌肝转移灶旁相对正常肝组织与正常肝组织MIF表达阴性。(4)Logistic回归分析 结果显示，大肠癌患者血清MIF水平是影响大肠癌肝转移的独立危险因素(OR=1．245，OR的 95％C／为1．017—1．524，P=0．034)。结论 MIF在大肠癌肝转移中可能发挥着重要的作用。 【关键词】 结直肠肿瘤； 巨噬细胞游走抑制因子；肝转移 The relationship between MIF expression and hepatic metastasis in patients of colorectal carcinoma HE Xing—xiang‘，LI Xiao一 u HA0 Yuan—tao，WEN Zhuo-fu，HUANG s zhang，sU Hang， CHEN De． Department of Gastroenterology，the Second Affiliated Hospital of Guangzhou Medical College。 Guangzhou 510260．China Corresponding author：CHEN De，E—mail：drchende@public．guangzhou．gd．en 【Abstract】 objective To determine the expression of maerophage migration inhibitory factor (MIF)in hepatic metastasis of colorectal carcinoma(CC)，colorectal carcinoma tissue and the serum level of MIF in patients with CC． Methods MIF expression was determined by immunohistochemical staining in 49 colorectal carcinoma specimens．16 corresponding adjacent nontumorous tissues．10 hepatic metastatic tumor tissues．and normal hepatic tissues and control healthy hepatic tissues in 5 cases．Enzyme—linked immunosorbent assay(ELlSA)was used to detect the level of MIF in the sera of 3O CC patients and 17 heahhy volunteers．The correlation between the serum expression of MIF and clinical factors of CC was tested by Logistic regression． Results In liver metastasis group，MIF expression in CC tissue and the level of MIF in serum wer