应用二次通用旋转组合设计优化黄绿绿僵菌固相培养基配方方案.PDFVIP

安徽农业大学学报, 2013, 40(4): 649-655 Journal of Anhui Agricultural University 网络出版时间：2013-7-8 18:08:22 [URL] /kcms/detail/34.1162.S1808.005.html 应用二次通用旋转组合设计优化黄绿绿僵菌固相培养基配方 * 徐阿妹，林华峰 ，李茂业，王 慧 (安徽农业大学植物保护学院，合肥 230036) 摘 要：在真菌孢子粉大规模生产中，筛选生防真菌最佳产孢培养基是提高产孢量的先决条件。选用廉价易得 的实验材料作固体培养基，在单因素试验的基础上，采用二次通用旋转组合设计，对影响黄绿绿僵菌 Mf82 菌株固 相发酵阶段产孢量的固体培养基组分及其配比进行了优化，并检测了所获孢子粉的质量指标。结果表明，单因素试 验，从大米粉、面粉、玉米粉、豆角壳和黄粉虫蜕中选择面粉、豆角壳与黄粉虫蜕以 4:1:0.2 配比混合作为固体培 养基配方时，该菌产孢量最高；优化实验，固相培养基中面粉、豆角壳和黄粉虫蜕的配比为 4:1:0.25 时，该菌产孢 量高达 2.39 ×1012 个·kg-1 ；孢子粉的指标检测，显示用该培养基配方生产的黄绿绿僵菌孢子粉达到了真菌孢子粉生 产的企业标准。 关键词：黄绿绿僵菌；固相培养；二次通用旋转组合设计；指标检测 中图分类号：S476.12 文献标识码：A 文章编号：1672−352X (2013)04−0649−07 Optimization of Metarhizium flavoviride solid phase medium formula by quadratic general rotary unitized design XU A-mei, LIN Hua-feng, LI Mao-ye, WANG Hui (School of Plant Protection, Anhui Agricultural University, Hefei 230036) Abstract: It is the precondition for improving conidia production to screen the best conidia medium for biocontrol fungi during the mass production of fungal conidia. In this study, cheap and convenient experimental materials were used to make solid culture medium, and on the basis of single factor experiment, quadratic gen- eral rotary unitized design was used to optimize the composition and proportion of solid medium which influ- enced solid fermentation stage conidia production amount of Metarhizium flavoviride Mf82, and the quality indicators of conidia powder were further tested. Results showed that in single factor experiment, conidia pro- duction of Metarhizium flavoviride Mf82 was of the highest amount, when flour, be