pSecTag2A哺乳动物表达载体说明.pdfVIP

pSecTag2 A 编号 载体名称 北京华越洋生物VECT6096 pSecTag2 A pSecTag2A 载体基本信息 载体名称: pSecTag2 A 质粒类型: 哺乳细胞表达载体；分泌表达载体 高拷贝/低拷贝: 高拷贝 启动子: CMV 克隆方法: 多克隆位点，限制性内切酶 载体大小: 5159bp 5 测序引物及序列: T7 -F：5d[TAATACGACTCACTATAGGG]3 3 测序引物及序列: -- 载体标签: 6X His, myc 载体抗性: Ampicillin 筛选标记: Zeocin 备注: 含有前导序列IgK，引导重组蛋白分泌表达。 稳定性: 瞬表达 组成型: 组成型 病毒/非病毒: 非病毒 pSecTag2A 载体质粒图谱和多克隆位点信息 pSecTag2A 载体简介 pSecTag2 and pSecTag2/Hygro are mammalian expression vectors designed for the secretion, purification, and detection of fusion proteins. Each vector has a large multiple cloning site in three reading frames to simplify cloning in frame with the N-terminal secretion signal. The vectors (Figure 1) offer the following features: Secretion signal from the V-J2-C region of the mouse Ig kappa-chain for efficient secretion of recombinant proteins (Figure 2) Cytomegalovirus (CMV) promoter for high-level constitutive expression C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody C-terminal c-myc epitope for detection with an Anti-myc Antibody Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence to enhance mRNA stability SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (e.g., COS-1, COS-7) The pSecTag2 vectors carry the Zeocin resistance gene for cost-effective selection in mammalian ce