高效液相色谱法测定健脾益肾颗粒中补骨脂素及异补骨脂素含量.docVIP

高效液相色谱法测定健脾益肾颗粒中补骨脂素及异补骨脂素含量[摘要] 目的：建立高效液相法测定健脾益肾颗粒中补骨脂素和异补骨脂素含量的方法。方法：采用Agilent C18色谱柱（250 mm×4.6 mm，5 μｍ），流动相为乙腈-水（53∶47），检测波长为246 nm，流速为1.0 ml/min，柱温为25℃。结果：补骨脂素在0.040 10～0.204 80 μg范围内呈良好的线性关系，回归方程为Y=80.05X+1.452（r=0.999 9）；平均回收率为99.8%，RSD=1.00%。异补骨脂素在0.034 22～0.212 00 μg范围内呈良好的线性关系，回归方程为Y=78.26X+2.653（r=0.999 9）；平均回收率为100.1%，RSD=0.87%。结论：本法操作简便，准确，重现性好，可用于健脾益肾颗粒的质量控制 [关键词] 健脾益肾颗粒；补骨脂素；异补骨脂素；高效液相色谱法 [中图分类号] R969.4 [文献标识码] A [文章编号] 1673-7210（2011）12（a）-070-03 Determination of Psoralen and Isopsoralen in Jianpi Yishen Granula by HPLC LIU Jun′e1, LIU Junhong2, ZHAO Tieyan3 1.Department of Nutrition, the Military General Hospital of Beijing, Beijing 100700, China; 2.Department of Blood Transfusion, 264th Hospital of Taiyuan, Shanxi Province, Taiyuan 030001, China; 3.Department of General Surgery, 264th Hospital of Taiyuan, Shanxi Province, Taiyuan 030001, China [Abstract] Objective: To establish an HPLC method for determining the contents of Psoralen and Isopsoralen in Jianpi Yishen Granula. Methods: Agilent C18 column (250 mm×4.6 mm, 5 μｍ) was used with acetonitrile- water (53∶47) as the mobile phase, the detective wavelength was at 246 nm, column temperature was 25℃. Results: There was a good linear relationship for Psoralen and Isopsoralen with the range of 0.040 10-0.204 80 μg, Y=80.05X+1.452 (r=0.999 9), and 0.034 22-0.212 00 μg, Y=78.26X+2.653 (r=0.999 9). The average recovery was 99.8% (RSD=1.00%) and 100.1% (RSD=0.87%) respectively. Conclusion: The methods is simple, accurate and repeatable. It may be practical to control the quality of Jianpi Yishen Granula. [Key words] Jianpi Yishen Granula; Psoralen; Isopsoralen; HPLC 健脾益肾颗粒为中药复方制剂，由补骨脂、党参、枸杞子、女贞子、菟丝子和白术等多味中药组成，具有健脾益肾的功效，能提高机体免疫力、抑制肿瘤的双向调节作用，减轻放化疗等副作用以及减轻脾肾虚弱所引起的疾病。补骨脂为处方中的君药，其有效成分为补骨脂素和异补骨脂素。文献中多采用高效液相色谱法[1-2]、气相色谱法[3]、薄层扫描法[4]和毛细管电泳法[5]等来测定其有效成分的含量。为了更有效地控制健脾益肾颗粒的质量，本文采用高效液相色谱法测定健脾益肾颗粒中补骨脂素和异补骨脂素的含量，并进行学研究，简便可靠，重复性好，能有效控制产品的质量 1 仪器与试药 Agilent 1200液相色谱仪（美国安捷伦科技有限公司，包括：四元泵、在线脱气