纳米金壳热疗治疗肝癌.doc

近红外激光诱导纳米金壳热疗治疗人肝癌HepG2细胞的实验研究 曲华伟 刘慧玉 张阳德 【摘要】 目的 研究在近红外激光的诱导下纳米金壳热疗对肝癌HepG2细胞的治疗作用。 方法 光学显微镜、电子显微镜观察细胞形态，四甲基偶氮唑蓝（MTT）比色法检测纳米金壳对HepG2肝癌细胞株的抑制率，进一步测定不同浓度纳米金壳热疗对肝癌细胞生长抑制的情况并进行比较，流式细胞仪（FCM）检测凋亡细胞。 结果 HepG2肝癌细胞经纳米金壳热疗作用后，细胞增殖受到明显抑制，细胞凋亡率明显增加，且与纳米金壳浓度呈依赖关系。 结论 纳米金壳热疗能抑制肝癌HepG2细胞增殖，促进其凋亡，对肝癌的治疗有一定的作用。 【关键词】纳米金壳 ；近红外光 ；癌 ；肝细胞 Study on the therapeutic effect of near-infrared mediated gold nanoshell hyperthermia on liver cancer HepG2 cell. QU Hua-wei*,ZHANG Yang-de, TANG Fang-qiong, HE Jian-tai,WANG Ji-wei,LIU Hui-yu. *National Hepatobiliary ＆ Enteric Surgery Research Center of South University,Changsha 410008,China Corresponding author : ZHANG Yang-de 【Abstract】Objective To study the therapeutic effect of near-infrared mediated nanoshell hyperthermia on human Hepatocarcinoma HepG2 cells in vitro and in vivo. Methods By light and electron microscopy，the morphologic changes of cells were observed . To detect the depression rate of gold nanoshell on human hepatocellular carcinoma HepG2 strain though MTT colorimetric method and to determine further the depression rate of different concentration of gold nanoshel to contract.Ratio of apoptotic cell was detected by flow cytometry. Results Near-infrared mediated gold nanoshell hyperthermia could significantly inhibit the proliferation and rapidly increase the ratio of apoptosis of HepG2 cells and the effect was dose-dependent，and could also induce a G2／M phase arrest in this cell line. Conclusion Near-infrared mediated gold nanoshell hyperthermia could inhibit the proliferation and promote apoptosis of human hepatocarcinoma HepG2 cells in a concentration-dependent manner. It could take apart role in the treatment of liver cancer. 【Keywords】Gold nanoshell；Near-infrared light ；Cancer；hepatocellular 肝癌是世界最常见的恶性肿瘤之一，其死亡率非常高。我国肝癌死亡已居农村恶性肿瘤之首位，在城市中仅次于肺癌而居第2位。传统的手术、放疗、化疗等都不能对其彻底治愈。因此，研究探索新疗法具有重要意义。近年来，随着纳米技术的研究进展，在纳米水平上研制出的纳米金壳材料引起了人们的广泛兴趣。纳米金壳是一种用惰性金属金包覆二氧化硅Hirsch［1］研制出纳米金壳，通过在近红外激光诱导照射下热疗治疗肿瘤取得了一定进展。我们运用自制