组学相关Identification of a regulation network in response to cadmium toxicity using blood clam Tegillarca granosa as model.pdfVIP

/scientificreports OPEN Identification of a regulation network in response to cadmium toxicity using blood clam Tegillarca Received: 27 April 2016 granosa as model Accepted: 04 October 2016 Published: 20 October 2016 1 2 3 4 1 3,† 1,† Yongbo Bao , Xiao Liu , Weiwei Zhang , Jianping Cao , Wei Li , Chenghua Li Zhihua Lin Clam, a filter-feeding lamellibranch mollusk, is capable to accumulate high levels of trace metals and has therefore become a model for investigation the mechanism of heavy metal toxification. In this study, the effects of cadmium were characterized in the gills of Tegillarca granosa during a 96- hour exposure course using integrated metabolomic and proteomic approaches. Neurotoxicity and disturbances in energy metabolism were implicated according to the metabolic responses after Cd exposure, and eventually affected the osmotic function of gill tissue. Proteomic analysis showed that oxidative stress, calcium-binding and sulfur-compound metabolism proteins were key factors responding to Cd challenge. A knowledge-based network regulation model was constructed with both metabolic and proteomic data. The model suggests that Cd stimulation mainly inhibits a core regulation