大白菜功能基因编码区SSRs分布规律探究.docVIP

大白菜功能基因编码区SSRs分布规律探究摘要：利用数据库中大白菜的部分基因组序列及其注释结果，对大白菜功能基因编码区分布的SSRs类型进行了分析。结果表明，编码区的SSRs以3核苷酸重复的最多，其次为6核苷酸重复的；各种基序的SSRs类型在基因编码区分布的数量有很大差异；另外编码氨基酸的三核苷酸SSRs在11 944个基因编码区前100 bp、中部及后100 bp的分布也有较大差异。说明大白菜基因编码区的SSRs具有相位和极性，其原因在于编码氨基酸的需要。正是这种相位和极性引起大白菜基因编码区SSRs分布的不均一性 关键词： SSRs；分布；基因；编码区；大白菜 中图分类号：S436.34 文献标识码：A DOI编码：10.3969/j.issn.1006－6500.2011.06.001 Distribution Patterns of Simple Sequence Repeats in Coding Regions of Genes in Chinese Cabbage ZHOU Xin-cheng, LI Li-bin, LIU Li-feng, LI Hua-yin, GAO Jian-wei (Institute of Vegetables, Shandong Academy of Agricultural Sciences, Shandong Key Laboratory of Greenhouse Vegetable Biology Jinan,Shandong 571101, China) Ａｂｓｔｒａｃｔ: Using the genomic sequences and the annotation information from public genome database of Chinese cabbage, the distribution patterns of SSRs in coding regions of genes were studied. It was found that tri-nucleotide repeat motifs were the most SSR types in coding regions of Chinese cabbage, with hexa-nucleotide motifs following. Much distinction was found for the distribution frequencies of SSR types with different repeat motifs in gene coding regions. And there was much difference for the distributions of SSRs types which encode amino acid in the first 100 bp, middle region and the last 100 bp of 119 44 genes. These distribute patterns reflected the phase and polar characteristics of SSRs in gene regions which could be attributed to encoding needs. It was concluded that the distribution of SSRs in coding regions of Chinese cabbage genes were un-randomly. Key words: SSRs; distribution; gene; coding regions; Chinese cabbage SSRs（Simple Sequence Repeats）也叫微卫星DNA，是由1～6个核苷酸组成的基序串联重复序列，广泛分布于动植物基因组中。已有的资料表明在植物基因组不同区域SSRs并不是均匀分布的 [1-2]。了解一个物种中SSRs的分布规律不仅对SSRs引物开发、比较基因组学的研究有指导意义，对于了解基因组进化以及SSRs的功能都有重要意义[3-5]。国内学者曾对黄瓜[6]、葡萄[7]、香菇[8]等植物基因组及基因区的SSR分布进行了研究，表明不同植物基因组上SSR的分布不仅不均匀，而且有各自特点 大白菜（Brassica rapa ssp. pekinensis，AA基因组，2n=20）为十字花科芸薹属植物，原产于中国，现广泛栽培于中国、韩国、朝鲜及日本。因其营养丰富且富含膳食纤维，人们普遍喜欢食用，在中国以