烤烟基因型间香味物质的差异分析.pdfVIP

6 中国烟草科学 2007，28(6)：6-8 烤烟基因型间香味物质的差异分析 王 霞，翟争光，杨铁钊 ，景延秋，史宏志，杨志晓，马俊峰 (河南农业大学，郑州 450002) 摘 要：对高香气烤烟新品系“8306”等8个烤烟基因型进行香味成分的差异分析，旨在显示“8306”的高香气特性。用分光 光度法测定叶面分泌物含量和残余法测定浸提后石油醚提取物的含量，用气相色谱／质谱联用仪 (GC／MS)对“8306”和中烟 100的香味物质含量和组成进行鉴定。结果表明，“8306”的叶面分泌物、石油醚提取物含量和总香值均较高，这可能是与其 它基因型香气质量差异的主要原因。“8306”的20种含量较高成分可能是其高香气产生的主要物质基础。“8306”的总香气成 分含量远高于中烟100，这可能是其香味突出的原因之一。 关键词：烤烟；叶面分泌物；石油醚提取物；香味物质 中图分类号：$572；TS41l 文献标识码：A 文章编号：1007．5119(2007)06．0006．03 Differences in Aroma Components among Flue—cured Tobacco Genotypes WANG Xia，ZHAI Zhengguang，YANG Tiezhao，JING Yanqiu，SHI Hongzhi，YANG Zhixiao，MA Junfeng (Henan Agricultural University,Zhengzhou 450002，China) Abstract：For expressing the superior aroma of new flue—cured line 8306，the experiments were conducted to compare the flavor components of eight flue—cured genotypes，with quantitative determination of leaf surface trichome exudate by spectrophotometry and the petrolum ether extract by residual method．and the identification of flavor components in“8306’’and Zhongyan1 00 by GC／MS．The result showed that 8306 had high contents of leaf surface trichome exudate，petrolum ether extract and total volulme of aroma．which IIlight contribute to the specific aroma quality of 8306．TIle twenty components in 8306 IIlight be the precursor of hilgh level of flavor．Th e total aroma components in 8306 was much higher than in Zhongyan1O0，which might partially result in the prominent aroma in 8306． Keywords：flue．cured tobacco；trichome exudate of leaf surface；petrolum ether extract；flavor components 烟草香味是评价烟叶质量的核心内容。烟叶中 许多因含量太低，难以确定其对香气的贡献，致使 许多挥发性、非挥发性香味物质对香气质、香气量 以提高香气质量为目标的育种研究进展缓慢IoJ。近 等有重要影响f】0】，这些物质含量不仅与环境有关， 年来，笔者从改良烟草腺毛分泌物入手，把提高烟 更取决于基因型的差异。如何在育种改良中有效地 面致香物质含量作为培育高香气品种的一