干旱胁迫对山葡萄左山二幼苗叶片光系统Ⅱ活性影响.doc

干旱胁迫对山葡萄左山二幼苗叶片光系统Ⅱ活性影响摘 要: 为深入研究干旱胁迫对山葡萄影响的生理机理和指导干旱地区山葡萄的生产栽培，以左山二的当年生扦插苗为试材，利用快速叶绿素荧光诱导动力学曲线分析技术研究了干旱胁迫下山葡萄幼苗叶片的光系统活性。结果表明，随着干旱胁迫的加重，F0上升，Fv/Fm、PIABS及ψ0降低。JIP-test分析发现随着干旱胁迫的加剧叶绿素荧光诱导动力学曲线中J、I点升高，并出现明显的K点；同时反映单位面积叶片光能利用率的ABS/CS、ET0/CS等参数下降，而单位反应中心的性能有所增加，热耗散增加。说明严重的干旱胁迫使山葡萄叶片PSⅡ电子传递链供体侧和受体侧均受到伤害，抑制了光合电子传递，导致产生过量激发能，最终导致光合作用的下降 关键词: 山葡萄； 干旱胁迫； 光系统Ⅱ 中图分类号：Ｓ６６3．1 文献标识码：Ａ 文章编号：１００９－９９８０?穴２０11?雪０6－977－０7 Effects of drought stress on activities of photosystems Ⅱ in leaves of young plants of amur grape Zuoshan 2 CHEN Li， WANG Zhen-xing， AI Jun*， XU Pei-lei， QIN Hong-yan， ZHAO Ying， LIU Ying-xue， JIAO Zhu-qing， SHEN Yu-jie （Institute of Special Wild Economic Animal and Plant Science， CAAS， Jilin 132109 China） Abstract: Activities of photosystem Ⅱin leaves on Zuoshan 2 annual cutting shoots under drought stress were studied by analyzing chlorophyll fluorescence transient for lucubrating the physiological mechanism of the effect of drought stress on amur grape and guiding amur grape culture in arid region. The results showed that F0 increased and Fv/Fm， PIABS，ψ0 decreased with increasing drought， and J and I steps increased and K step appeared evidently. Meanwhile， light use efficiency per unit leaf area expressed by ABS/CS，ET0/CS and other parameters decreased while capacity per reaction center increased and dissipated energy flux increased under drought stress. The results showed that the damage of both donor side and acceptor side of PSⅡin leaves of Amur grape under drought stress could cause the inhibition of electron transportation and the accumulation of excessive excited energy， which finally result in the decrease of photosynthesis . Key words: Amur grape； Drought stress； Photosystem Ⅱ 世界上限制农作物产量的诸多环境因素中，最重要的是供水条件，全世界有1/3的可耕地处于供水不足的状态，而其他耕地也常会遭受周期性的干旱，世界性的水分亏缺导致的减产超过其他因素所造成减产的总和[1]。山葡萄（Vitis amurensis Rupr）为葡萄科葡萄属落叶藤本果树，原产于我国东北、俄罗斯远东地区和朝鲜半岛，因其较高的经济价值在我国东北及内蒙古地区栽培面积很大。近年来随着栽培区域的扩大，山葡萄栽培逐渐向干旱和半干旱地区发展，选育耐旱性品种及采取相应的栽培技术措施对山葡萄产业的发展具有重要意义 植物的光合机构