大鼠原位肝移植的技术改进_李涛.pdf

14 7 V ol. 14 N o. 7 2005 7 Chinese Journal o f G eneral Surg ery Ju l. 2005 : 1005- 6947 ( 2005 07 - 0486- 03 1 2 1 1 李涛 , 唐华美, 孙星 , 彭志海 ( 上海市第一人民医院 1. 普外科 2. 病理科, 上海 200080 : ( OLT , ; SD SD, SD W istar 30 , 1 5m inSD SD 3 9 7% ; SD W istar 8~ 15 d, , , SD W istar : ; ; , : R 65 7. 1; R 36 3 : A Technical improvement of two-cuffmethod of orthotopic liver transplantation in rats 1 2 1 1 L I T ao , TANG H ua-m e i , SUN X ing , PENG Zh i- ha i ( 1. D ep ar tm ent of G eneral Surg ery 2. D ep ar tm ent of P atho logy, Shang ha i F irst P eop le s H o sp ital, Shang ha i 2 00 0 8 0, Ch ina ) Abstract: Objective T o investiga te an mi proved technique o f o rtho top ic liver transp lantation w ith the tw o- cuffm e thod in rats, and to observe allograft rejection after transplanta tion. Methods 30 W istar rats w ere grafted w ith livers of SD ra ts and 3 0 SD rats w ere grafted w ith livers o f SD ra ts by u sing a mod ified wt o- cuff techn ique and exam ined for po stopera tive rejection. Results T he anhepatic pe riod w as about 15 m inutes. The 2 4- hour surv ival rate w as 10 0% . The W istar rats grafted w ith SD livers d ied 8 to 1 5 days after liver transplantation, and the histopatho log ic exam ination show ed var iou s deg rees of allog ra ft rejection. T he SD ra ts grafted w ith livers o f SD rats had a surv iv al rate of 97% at 3 w eeks postoperative ly. Conclusions The m odif ied wt o- cuff techn ique