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摘要-学位论文管理系统-暨南大学
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Abstract
Objective To investigate whether the low dose ultraviolet A is harmful or not to normal
human facial skin. It provides basis for people to protect from photoaging effectively in
daily life.
Materials and Methods Skin specimens from 14 healthy individuals were obtained after
the “eyebag removal” operation (from July 2005 to January 2006). The right skin
specimens were irradiated by UVA of 1mw/cm2 in low temperature for one hour. The left
skin specimens were placed in the same condition covered with silver paper. Removed
the epidermis after immersed in Dispase II and Collagenase I solution respectively.
Extracted the total cellular DNA of fibroblasts and ran nested-PCR. Scanned the gel into
computer, detected the band images, transformed them into figures with affiliated
software and calculated the frequency of mitochondrial DNA common deletion.
SPSS10.0 were used to achieve final statistic results.
Results The frequency of mitochondrial DNA common deletion in UVA-exposed cells is
significantly higher (0.2850.193) than that of UVA-unexposed cells (0.0980.175)
with t=4.793 and P0.05. The frequency of mitochondrial DNA common deletion in
norm
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