The Sulfolobus solfataricus AAA protein Sso0909,：（的硫化叶菌solfataricus AAA蛋白质Sso0909,）.pdfVIP

94 Biochemical Society Transactions (2008) Volume 36, part 1 The Sulfolobus solfataricus AAA protein Sso0909, a homologue of the eukaryotic ESCRT Vps4 ATPase ´ 1 Cedric F.V. Hobel*, Sonja V. Albers†, Arnold J.M. Driessen† and Andrei N. Lupas* *Max-Planck Institute for Developmental Biology, Department of Protein Evolution, Spemannstrasse 35, 72076 Tubingen,¨ Germany, and †Department of Molecular Microbiology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands Abstract Sso0909 is a protein of the thermo-acidophilic crenarchaeon Sulfolobus solfataricus, annotated as a p60 katanin-like ATPase. We present here results supporting the hypothesis that Sso0909 is an orthologue g of the eukaryotic ESCRT (endosomal sorting complex required for transport) ATPase Vps4 (vacular protein r sorting 4). The spectrum of Sso0909 homologues is limited to several orders of Crenarchaea and to three o. euryarchaeal Thermoplasmata species, where they were presumably acquired by lateral gene transfer. s Almost invariably, Sso0909 homologues occur in the genomic vicinity of homologues of eukaryotic ESCRT-III n components, which are the targets of disassembly by Vps4, as well as with a creanarchaeal-specific a coiled-coil protein. S. solfataricus sso0909 is constitutively expressed under normal growth conditions and r t appears to be essential, as judged by the failure to obtain stable deletion mutants. We expressed Sso0909 c o