Yeast Bim1p promotes the G1-specific dynamics of microtubules：（酵母Bim1p促进微管的G1-specific动态）.pdfVIP

Yeast Bim1p Promotes the G1-specific Dynamics of Microtubules ‡ § § Jennifer S. Tirnauer,* Eileen O’Toole, Lisbeth Berrueta,* Barbara E. Bierer, and David Pellman * *Department of Pediatric Oncology, Dana-Farber Cancer Institute, §Department of Pediatric Hematology, The Children’s ‡ Hospital, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115; and Boulder Laboratory for 3D Fine Structure, Boulder, Colorado 80309 Abstract. Microtubule dynamics vary during the cell EB1, Bim1p localizes to dots at the distal ends of cyto- cycle, and microtubules appear to be more dynamic in plasmic microtubules. This localization, together with vivo than in vitro. Proteins that promote dynamic insta- data from electron microscopy and a synthetic interac- bility are therefore central to microtubule behavior in tion with the gene encoding the kinesin Kar3p, suggests living cells. Here, we report that a yeast protein of the that Bim1p acts at the microtubule plus end. Our in highly conserved EB1 family, Bim1p, promotes cyto- vivo data provide evidence of a cell cycle–specific mi- plasmic microtubule dynamics specifically during G1. crotubule-binding protein that promotes microtubule During G1, microtubules in cells lacking BIM1 showed dynamicity. reduced dynamicity due to a slower shrinkage rate, fewer rescues and catastrophes, and more time spent in Key words: cell cycle • cell polarity • microtubule- an attenuated/paused state. Human EB1 was identified associated proteins • microtubules • Saccharomy