水稻单片段代换系对两种不育细胞质恢复性的遗传分析论文.pdfVIP

中国水稻科学((?hinJRices(i)，2013，27(2)：145—152 http }Ⅶww．ricesci．cn DOI：10．3969／j．issn．100l7216．2013．02．006 145 水稻单片段代换系对两种不育细胞质恢复性的遗传分析 蔡健 代资举。 朱海涛 曾瑞珍z 张泽民z 王敉敉 马同富 张桂权z， (1阜阳师范学院 生命科学学院，安徽 阜阳 236041；2华南农业大学 植物分子育种重点实验室，广东 广州 510642；*通讯联系人， Email：gqzhang@scau．edu．cn；matongfu6328@ 126．corn) GeneticAnalysisofRestoration ofChromosomeSingleSegmentSubstitution IinetotwoDifferentCM SLinesinRice CA1Jian ，DAIZi—ju ，ZHU Hai—tao ，ZENGRui—zhen。。ZHANGZe—min ，WANGMimi，MATong—fu一 ， ZHANG Gui—quan2， (1SchoolojL Sciences，FuyangTeaehersCollege，Fuyang236041，China； 2StateKey LaboratoryforConservationand UtilizationoJ SuhtropitalAgro—bioresour~es，SouthChinaAgriculturalUniversity，Guangzhou510642，China； C(’rrsp(}nding author，E-mail：gqzhan @scau．edu． ；matongJh6328@126．CO?TI) CAIJian，DAIZiiu，ZHU Haitao，eta1．Geneticanalysisofrestorationofchromosomesinglesegmentsubstitutionline totwodifferentCMSlinesinrice．ChinJRiceSci，2O13，27(2)：l45l52． Abstract：A ricechromosomesinglesegmentsubstitution 1ine(SSSI)$42，which isastrongrestorer lineforwild abortivecytoplasmicmalesterility(W A—CMS)andY CMS。wasidentifiedattheGuangdongProvineialKeyIaboratory ofPlantMolecularBreeding，SouthChinaAgriculturalUniversity．Thegeneticmodeoffertilityrestorer(RJ)genes andthegeneticrelationship between W A—CMS and ’ CMS systemswerecharacterized through theproduction of backcrosspopulations． The SSS1 S42 wasused to pollinate WA—CMS line ofZhenshan 97A and Y—CMS 1ineof Y—HuanongA andgeneratedtwoBC F2populationsbyusingmarker—assisted selectionandtraditionalbackcrossing． Resultswereshowtlasthefollowing：1)Inthegeneticbackgroundof$42，W A_CMSwasrestoredmoreeasilythan Y—CMS，andtheeffectofR_，4appearedtobeslightlylargerthanthatofR． f3forthetwoCMSsystems；2)Twopairs ofdominantgenesRJ3andR governedpollenfertilityrestoration，andmodifyingorminorgenesalsoin